豆みそPeptideのPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPeptideに関する研究(第10報)

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The growth promotion of molecular sive fractions obtained from soybean miso for Pediococcus halophilus was studied.1. Growth promotion for Pc.halophilus, Pc. acidilactici, and Streptococcus farcalis was observed after the supplement of molecular sieve fractions to the synthetic medium.The growth promotion of lower molecular weight fractions (C-25,X-16) was higher than that of the higher molecular weight fraction (X-4). These effects were most remarkable in the case of Pc.halophilus.2. General characteristics of the growth-promoting substances in X-16 were as followes. (1). Electrophoretic behavior of the growth promoter was similar to that of neutral amino acids. (2). The R_1 value on paper partition chromatography was nearly 0.25 between those of alanine and proine in a butanol-acetic acid-water(4 : 1 : 5) system. (3). Better growth was observed by the addition of the neutral and basic fraction which was separated on Amberlite IR-4B (Cl, pH 3.50) column.3. Two growth-promoting substances (tentatively named B-fr. and P-fr.) which could be separated on an Amberlite IRA-402 (OH) column existed in X-16.B-fr. was a basic substance and half-maximal growth of Pc.halophilus was observed, after its addiition. However, maximal growth was not observed after the addition of B-fr. with betaine.P-fr. was an amphoteric substance (peptidic substance). The additon of P-fr. alone had almost no effect on the growth of Pc. halophilus. However, the addition of P-fr. resulted in maximal growth in the presence of betaine or B-fr.
社団法人日本生物工学会の論文
1973-03-25

豆みそPeptideのPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPeptideに関する研究(第10報)

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