豆みそ塩基性物質 (B-fr.) のPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPrptideに関する研究 (第13報)

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B-fr. and P-fr., two growth promoting fractions for Pc. halophilus, were studied by separation from defatted soybean flour, three kinds of koji, soybean miso and so on.1. B-fr. obtained from soybean miso was more stimulative toward growth of Pc. halophilus than betaine, and was composed of two growth promoting substances. That is, the main growth promoting substance in B-fr. corresponded to betaine, the other was unkinown basic substance having a slight effect on growth.2. Growth promoting activity of betaine in the presence of P-fr. was higher than that of choline.3. B-fr., P-fr. were present in autoclaved soybean flour, three kinds of koji (rice, wheat bean, defatted soybean) obtained from pure culture with Asp. sojae, and in the hydrolyzate pf defatted soybean obtained by protease preparation from Asp. oryzae. Growth promoting activity of these P-fe. was increased remarkably by the proteolysis of soybean by koji, and by the protease preparation, and was higher than those of autoclaved soybean flour and polypeptone. However, there were no differences in the activity among B-fr. which were prepared from above materials.It is possible to consider on the basis of the above results that P-fr. controlled strictly the growth of Pc. halophilus in miso and soy-sauce, and that the remarkable stimulative activity of P-fr. resulted from the proteolytic characteristics of Asp. oryzae.
社団法人日本生物工学会の論文
1975-01-25