DipeptideのPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPeptideに関する研究(第15報)

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The effect of 59 synthetic dipeptides on growth of Pc. halophilus was studied using synthetic medium containing betaine.About 24 dipeptides slightly stimulated the growth of Pc. halophilus when added alone.Of these dipeptides, glycyl-L-serine, L-serylglycine and L-alanyl-L-valine exhibited copmparatively high growth promotion. However, on addition of a mixture of dipeptides which have a slight stimulative activity, growth promotion was more obvious and the lag time of Pc. halophilus was also reduced. Further, the growth of Pc. halophilus was more elevated by adding a mixture of a large number of dipeptides.On the other hand, growth promotion of Pc. halophilus by the low molecular weight peptides (P-fr.) in soybean miso was gradually reduced on removing some peptides from P-fr. by treatment on ion-exchange resin, Sephadex gel etc..All the above results are compatible with the conclusion that the growth promotion of Pc. halophilus by the low molecular weight peptides in soybean miso depends upon the cooperative action of several peptides having stimulative activity.L-Histidyl-L-histidine, L-histidyl-Ltyrosine and L-histidyl-L-phenylalanine inhibited the growth of Pc. halophilus at a concentration of 50 μg/ml, and dL-tyrosyl-L-tyrosine at over 100 μg/ml. However, the growth inhibitory effect of these peptides is probably not seen in miso and soysauce, because these are readily hydrolyzed by the proteases produced by Aspergillus oryzae.
社団法人日本生物工学会の論文
1976-03-25

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竹内徳男
愛知県食品工業試験所

DipeptideのPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPeptideに関する研究(第15報)

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