豆みその低分子Peptide区分(P-fr.)のPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPeptideに関する研究(第14報)

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The growth promoting substance in P-fr., a peptidic substance in the low molecular sieve fraction of soybean miso which promoted the growth of Pediococcus halophillus to maximal level in the presence of betaine was studied by separation on Sephadex G-15 and Dowex 50-X2 columns.1). For the growth promotion of Pc. halophilus by P-fr. it was necessary to add betaine, irrespective of the concentration of P-fr.. 2). By gel-filtration on Sephadex G-15,P-fr. was separated into three fractions which stimulated the growth in the presence of betaine. Of these, the peptide fraction(G-P) was most effective.3). Most peptide peaks separated from G-P by chromatography on Dowex 50-X2 ion-exchange resin exhibited a slight stimulative activity. However, comparatively high growth promotion was observed at the positions at which the major peptide of soybean miso is eluted on the chromatogram. Further, these major peptide fractions remarkably stimulated the growth of Pc. halophilus when a mixture of them was added.4). From the above results, it is possible to conclude that the remarkable growth promotion of Pc. halophilus by P-fr. in the presence of betaine depends upon the cooperative action of several peptides of slight stimulative activity, not upon the action of a single peptide, and that limiting peptides produced by the proteolytic characteristics of Aspergillus oryzae would promote effectively the growth of Pc. halophilus.
社団法人日本生物工学会の論文
1976-03-25

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竹内徳男
愛知県食品工業試験所

豆みその低分子Peptide区分(P-fr.)のPediococcus halophilusに対する増殖促進性 : みそ, しょう油のPeptideに関する研究(第14報)

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