Pediococcus soyae の培地組成について (第2報)

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In a previous report we confirmed that good growth of Ped. soyae was obtained in a medium containing soy-sauce.This report deals with further study made on the application of soy-sauce as a growth medium and on the effect of pH adjustment during the growth culture.The results obtained were as follows : 1) All the unpasteurized soy-sauces and soy-sauce koji extract were effective for the growth of Ped. soyae while chemically processed soy-sauce had little effect.2) Consequently, it was shown that the peptone-yeast extract medium, which has been usually regarded as the most suitable for growth of Ped. soyae, can be substituted with a soy-sauce medium.3) As was already known, good growth of Ped. soyae can generally be oibtained by maintaining pH in the medium higher than 5.5 during incubation.Better growth was observed in the medium with a larger buffer action.A much higher cell crop was obtained by raising pH of the medium during incubation higher than 5.8 by the addition of alkali since the buffer capacity of the medium was still insufficient after the addition of Na-succinate.4) In this experiment maximum growth indicated by the viable cell count was 10^9/ml in soy-sauce medium (total nitrogen 0.4%), when pH was raised higher than 5.8.
社団法人日本生物工学会の論文
1965-07-25