Pediococcus soyaeの培地組成について(第1報)

概要

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This research was done in an attempt to find the best medium for the cuuture of Ped.soyae by appropriate combinations of peptone, yeast extract, koji extract and soy sauce.1)1 % glucose was the optimum carbon source. And a good growth was observed in the medium containing 0.1-0.15M Na-succinate as a buffer.2)The full growth of Ped.soyae was not obtained in the medium containing peptone, koji extract or yeast extract (1-K) as a sole component. Yeast extrct (D, d) gave better results than the above 3 substances, but even then the best growth was not obtained. Only soy sauce (TN 0.2%) could make Ped. soyar reach full growth.3)The growth promoting factors of each substance were evaluated in synthetic media. Yeast extract (D) and soy sauce were the most effective of the substances tested.These materials contained significant growth stimulants for Ped. soyae which were active in the substances such as amins acids, minerals, vitamins, bases, betaine and peptides. However, the growth stimulant effect of yeast extract (1-k), koji extract and peptone was inferior to that of soy sauce and yeast extract (D).4)The growth was, to a certain extent, stimulated by a combination of peptone and yeast extract. But generally a combination of soy sauce and other substance was most effective for the growth promotion of Ped. soyae, and the maximum growth was obtained in the soy sauce media supplemented by yeast extract.
社団法人日本生物工学会の論文
1964-05-15