抗生物質の醗酵工業への応用 (第3報) : 酒精醪の汚染細菌が生産する抗酵母物質について

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The previous study at our laboratory indicated that No. B-14 strain isolated from alcohol mash produced an antiyeast substance and caused the decrease in the percentage of fermentation. This No. B-14 strain resembles B. subtilis very closely in its biological characteristics, and the antiyeast substance inhibited the growth of some bacteria.When the strain was grown under submerged condition in a medium containing sweet potato extract, peptone, beef-extract, glucose and NaCl, the antiyeast substance was found both in culture filtrate and in mycelium. During the shaking culture pH decreased to about 5.0 and then increased to 7.2,and the production of the antiyeast substance reached the maximum after 48 hrs.The culture broth was made to pH 2.0 with hydrochloric acid, and the precipitate formed was separated from the broth by filtering. The precipitate was extracted with methyl alcohol and the extract obtained was evaporated in vacuo at below 40℃. The residue was dissolved in a small amount of methyl alcohol and the antiyeast substance was precipitated with ethyl ether. The antiyeast substance was further purified by repeating chromatography with active alumina, and recrystallized from methyl alcohol and ethyl ether.On the other hand, the broth filtrate was stirred with 1% activated carbon for 30 min, and then the active agent adsorbed almost completely on activated carbon at pH 2.0 was eluted with methyl alcohol.The antiyeast substance was obtained from this methyl alcohol solution by the same method as described above. The crystalline reineckate of antiyeast substance was obtained from a rich aqueous solution of it by adding saturated aqueous solution of ammonium reineckate. The reineckate decomosed at 154〜160℃ and its elemental analysis was as followes : C 27.83%, H 4.43% N 22.20%, S 21.69%, Cr 10.52%. Methanol solution of the antiyeast substance had no maximum in the ultraviolet absorption; its ninhydrin test was positiive, but the biuret reaction, the SAKAHUCHI, the MOLISCH, and the MILLON tests as well as the ferric chloride test were negative. The crystalline reineckate of antiyeast substance inhibited the growth of yeast as follows : Haken No. 1 at 2 mg/ml, S. sake at 3mg/ml, Rasse XII at 3 mg/ml, Z. salsus at 2 mg/ml, T. utilis at 2mg/ml and C. albicans at 5mg/ml. This antiyeast substance is different in its properties from the already known antifungal substances produced by bacteria, so it is a new substance and named yeastcidin.
社団法人日本生物工学会の論文
1959-05-15

抗生物質の醗酵工業への応用 (第3報) : 酒精醪の汚染細菌が生産する抗酵母物質について

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