シアン含有の産業廃液処理 : (第4報) Fusarium salaniのシアン分解酵素系の精製と二, 三の特性について

概要

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Fusarium solani induces a cyanide-degrading enzyme system during the process of its adaptation to cyanide. In this reptort, the properties of the inducible cyanide-degrading enzymes of the two strains of F. solani were compared. Cyanide degrading enzymes of F. solani No.1 and No.2 strains were purified up to about 1500-fold and 400- fold respectively by DEAE-Sephadex chromatography and Sephadex G-75 gel filtration. The enzyme yield was 10%. The specific activities of these purified enzymes were of almost an equal value viz. 16〜17 l/mg protein hr. On the other hand, the specific activity of the intact cells of F. solani No.2 strain was greater tahn that of F. solani No.1 strain by 4〜5 times. Therefore, the authors supposed that the difference of the activities between the two srains derived from the differene of the cyanide degrading enzyme content.The molecular weighs of cyanide-degrading enzymes of F. solani No.1 and No.2 strains were 40,000 and 17,000 respectively. In both strains, the optimal pH, optimal temperature and activation energy of the nezyme reaction were 8.0,25〜30℃ and 12,000〜13,000 cal/mole, respectively.
社団法人日本生物工学会の論文
1969-10-25