清酒麹菌の研究(第7報) : 米麹の蛋白質分解力に就いて(其の1) : FOLIN-CIOCALTEUのphenol試薬に依る蛋白質分解力の測定

概要

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We determined the proteolytic activity with FOLIN-CIOCALTBU's reagent as follows : Casein was digested by proteolytic enzyme under a specific condition, and tyrosine and tryptophan contained in that part of digested casein, which was soluble in 0.2 mol. trichloroacetic acid, were measured by colorimetry using FOLIN-CIOCALTEU's reagent. The reagent was very stable under various conditions, and the standard curve showing the correlation of optical density (E) with the concentration of pure tyrosine and tryptophan was very reliable, and in this case the concentiation of trichloroacetic acid exerts no influence on the degree of colorization. We use for the development of colour a mixture of 1 cc of the original reagent (diluted five times), 1 cc of sample solution (containing less than 100γ of tyrosine) and 5 cc of 0.4 mol Na_2CO_3,keeping on a water bath at 37℃ for 20 min.
公益社団法人日本生物工学会の論文
1955-01-15