チャ赤焼病細菌Pseudomonas syringae pv. theaeの分離用培地(学術報告)

概要

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A new medium for the isolation of Pseudomonas syringae pv. theae was developed. This medium (PST-SM) contains 20 g of proteose peptone, 10 ml of glycerol, 1.5 g of disodium hydrogen phosphate, 1.5 g of magnesium sulfate, 250 mg of cephalexin, 100 mg of cycloheximide and 12 g of agar in 1000 ml of distilled water, and adjusted to pH 7.2. P. syringae pv. theae produced an opalescent colony, which was distinguishable from most bacteria tested (34 species in 4 genera) when incubated at 250℃ from 2 to 4 days. The most characteristic sign of the colony of the wild type strain of P. syringae pv. theae was recognized as a fried-egg type colony with granular and fibril structures when it was irradiated with light in an oblique downward direction from a light microscope at 2 days after incubation. Plating efficiency of this medium to P. syringae pv. theae was the same as King's B medium. R syringae pv. theae could be detected selectively from infected tea plant tissue with a selective limitation of more than 10^2 cfu per gram of tissue.
日本植物病理学会の論文
2002-08-25