A Highly Sensitive Assay for Proteases Using Staphylococcal Protein A Fused with Enhanced Green Fluorescent Protein(Analytical Chemistry)
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概要
- 論文の詳細を見る
Enhanced green fluorescent protein (EGFP) was fused with staphylococcal protein A (SpA) and used as a substrate for proteases. An SpA-EGFP assay was done in three steps : (i) digestion of SpA-EGFP by proteases, (ii) addition of rabbit IgG immobilized on Sepharose beads, and (iii) measurement of the fluorescence intensity of supernatant. The assay was sensitive enough to measure picogram levels of trypsin and chymotrypsin, and may be applicable to various other proteases as one of the most sensitive methods.
- 社団法人日本農芸化学会の論文
- 2002-07-23
著者
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AOKI Takashi
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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Aoki Takashi
School Of Health Sciences Kyushu University
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WATABE Hiroyuki
Department of Internal Medicine, Kitasato University School of Medicine
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Fujino Hiroaki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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FUJINO Hiroyoshi
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkai
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Watabe Hiroyuki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Aoki T
School Of Medicine Juntendo University
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WATABE Hiroyuki
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Watabe Hiroyuki
Department of Applied Chemistry, Faculty of Engineering, Yamagata University
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