Application of "Homogeneous Assay for Fluorescence Concentrated on Membrane" to the Analysis of the Substrate Specificity of Protease
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概要
- 論文の詳細を見る
The utility of the homogeneous assay for fluorescence concentrated on membrane (HAFCOM) in the analysis of the substrate specificity of protease was investigated using tobacco etch virus (TEV) protease. The Vmax of TEV protease against variants of a substrate was obtained by a simple procedure. It was considered that HAFCOM was more accurate than other endpoint measurements of protease assay.
- 社団法人 日本農芸化学会の論文
- 2010-04-23
著者
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NUNOME Kana
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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Tsuchida Shirou
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Yahara Taemi
Department Of Molecular Biosciences School Of Pharmaceutical Sciences Health Sciences University Of
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Hamaue Naoya
Department Of Molecular Biosciences School Of Pharmaceutical Sciences Health Sciences University Of
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Shigematsu Takashi
Department Of Molecular Biosciences School Of Pharmaceutical Sciences Health Sciences University Of
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Aoki Takashi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Molecular Biosciences School Of Pharmaceutical Sciences Health Sciences University Of
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Aoki Takashi
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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Aoki Takashi
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Shigematsu Takashi
Department of Information Processing, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Yokohama 226-8502, Japan
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