Application of Green Fluorescent Protein to Affinity Electrophoresis ; Affinity of IgG-Binding Domain C from Streptococcal Protein G to Mouse IgGl
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概要
- 論文の詳細を見る
Affinity electrophoresis(AEP)using green fluorescent protein(GFP)was studied. We constructed a fusion pprotein that linked S147PGFP and IgG binding domain C from streptococcal protein G (GFP-SpGC). The affinity of GFP-SpGC for mouse IgGl was measured. The AEP using GFP does not require a staining step after electrophoresis, and was successful with a non-purified sample. Therefore, this method is simple and useful for measuring many samples such as those used in mutational studies.
- 公益社団法人日本薬学会の論文
- 2002-02-01
著者
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YAMADA Keiko
Department of Ophthalmology, School of Medicine, Keio University
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Aoki T
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Watabe H
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Watabe Hiroyuki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Kazama Hitoshi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Yamada Keiko
Department Of Anatomy Hokkaido University School Of Medicine
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Aoki Takashi
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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Yamada Keiko
Department Of Anaomy Hokkaido University School Of Medicine
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Yamada Keiko
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Watabe Hiroyuki
Department of Applied Chemistry, Faculty of Engineering, Yamagata University
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