Biochemical and Immunochemical Properties of Modified Human Neuron-Specific Enolase
スポンサーリンク
概要
- 論文の詳細を見る
^<125>I-Labeled recombinant human neuron-specific enolase (R-NSE) was inadequate for RIA as a labeled antigen.The binding activity of laveled R-NES to the antibody was markedly decreased. To supplement this defect and facilitate purification, we constructed two R-NSE derivatives, Y-NSE (one tyrosine residue was added at the N-terminal of R-NSE) and Y-NSE.H6 (six histidine residues were further added at the C-terminal of Y-NSE). The biochemical and immunochemical characteristics of these R-NSE derivatives were essentially the same to those of R-NSE. These derivatives were useful not only as standards for enzyme immunoassay (EIA), but also as labeled antigens for RIA. These results clearly indicate that the reactivity of these modified NSEs to anti-NSE antibody is almost equivalent to that of human brain γγ-enolase (B-NSE), and that even if the modified NSEs are labeled, they retain their binding affinities to antibodies in contrast to R-NSE.
- 公益社団法人日本薬学会の論文
- 1997-05-15
著者
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AOKI Takashi
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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Aoki Takashi
School Of Health Sciences Kyushu University
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WATABE Hiroyuki
Department of Internal Medicine, Kitasato University School of Medicine
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Morikawa J
Eiken Chemical Co. Ltd.
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Morikawa Junji
Eiken Immunochemical Laboratory Co.
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ONAGI Hitoshi
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkai
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KANETA Mitsuhiro
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkai
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Watabe Hiroyuki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Onagi Hitoshi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Kaneta M
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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Aoki Takashi
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Aoki T
School Of Medicine Juntendo University
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WATABE Hiroyuki
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Watabe Hiroyuki
Department of Applied Chemistry, Faculty of Engineering, Yamagata University
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