Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease
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概要
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The utility of the homogeneous assay for fluorescence concentrated on membrane (HAFCOM) in the analysis of the substrate specificity of protease was investigated using tobacco etch virus (TEV) protease. The Vmax of TEV protease against variants of a substrate was obtained by a simple procedure. It was considered that HAFCOM was more accurate than other endpoint measurements of protease assay.
著者
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Tsuchida Shirou
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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Shigematsu Takashi
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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Yahara Taemi
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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NUNOME Kana
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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HAMAUE Naoya
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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AOKI Takashi
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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