A Flexible Single-Step Detection of Blotted Antigen Using a Fusion Protein between Protein A and Green fluorescent Protein
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概要
- 論文の詳細を見る
A green fluorescent protein mutant (S147P GFP) was fused with protein A and expressed in Escherichia coli. This fusion protein (PA-GFP147) was used in immunoblotting studies as a new detection system, designated as "flexible single-step detection (FSSD)". In FSSD, the detection of blotted antigen was done in one step, and the antigen-antibody reaction can be monitored by UV-irradiation in real time. The reaction time, therefore, can be flexibly controlled by monitoring the green fluorescence.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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AOKI Takashi
Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University o
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Aoki Takashi
School Of Health Sciences Kyushu University
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WATABE Hiroyuki
Department of Internal Medicine, Kitasato University School of Medicine
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Fujino Hiroaki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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FUJINO Hiroyoshi
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkai
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MIYASHITA Mamiko
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University
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Watabe Hiroyuki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Aoki Takashi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Miyashita Mamiko
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University
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Aoki Takashi
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Aoki T
School Of Medicine Juntendo University
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WATABE Hiroyuki
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Watabe Hiroyuki
Department of Applied Chemistry, Faculty of Engineering, Yamagata University
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