Rapid Degradation of the Silkworm Diapause Hormone by Trypsin and Its Suppression by VAP-map, a Synthetic Analog of the Cuticular Peptide of Silkworm, Bm ACP-6.7 (VAP-Peptide)
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概要
- 論文の詳細を見る
Very fast tryptic degradation of the silkworm diapause hormone was found and the degradation pathway was analyzed by moderating the reaction conditions. It proceeded via cleavage at Arg^<23> and finally at Arg^<15> of DH. As the C-terminal structure of DH was essential for exhibiting bioactivity, the first cleavage caused rapid inactivation of the hormone. This tryptic digestion was strongly suppressed by adding VAP-map, a synthetic analog of the cuticular peptide of silkmoths, Bm ACP-6.7 (VAP-peptide), which is a natural synergist of DH. VAP-map suppressed the enzymic reaction by interacting with the substrate, but not with the enzyme.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
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Yamashita Okitsugu
Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University
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Yamashita Okitsugu
Graduate School Of Bioagricultural Sciences Nagoya University
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Yamashita Okitsugu
Laboratory Of Sericultural Science
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Yamashita Okitsugu
Laboratory Of Sericultural Science Faculty Of Agriculture Nagoya University:(please Address)reprint
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KATSUZAKI Hirotaka
Faculty of Bioresources, Mie University
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Imai K
Faculty Of Bio-resources Mie University
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Imai Kunio
Mie Univ. Tsu Jpn
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Imai Kunio
Laboratory Of Organic Chemistry Faculty Of Agriculture Nagoya University
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Imai Kunio
Faculty Of Bioresources Mie University
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KOMIYA Takashi
Faculty of Bioresources, Mie University
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Komiya T
Mie Univ. Mie Jpn
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Komiya T
Mie Univ. Mie
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Baba Hitoshi
Faculty Of Bio-resources Mie University
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Komiya Takashi
Faculty Of Bio-resources Mie University
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Katsuzaki H
Faculty Of Bio-resources Mie University
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Imai Kunio
Faculty Of Bio-resources Mie University
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IMAI Kunio
Faculties of Bioresources, Mie University
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Katsuzaki Hirotaka
Faculty of Bio-resources, Mie University
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KOMIYA Takashi
Faculties of Bioresource, Mie University
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IMAI Kunio
Faculties of Bioresource, Mie University
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KATSUZAKI Hirotaka
Faculties of Bioresource, Mie University
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