Isolation and Structure of Diapause Hormone of the Silkworm, Bombyx mori.

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概要

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• The embryonic diapause of the silkworm, Bombyx mori is induced by a neuropeptide hormone, diapause hormone, which is secreted from the suboesophageal ganglion. About 55, 000 complexes of the suboesophageal ganglion and the first thoracic ganglion were dissected out from day 1 pupae of silkworms. The hormone was extracted with hot water and purified by a reverse-phase high performance liquid chromatography on a TSK gel octadecyl-4PW column. The second chromatography gave a single symmetric peak and the isolated hormone induced diapause in 20% eggs at a dose of 1.5ng/pupa. Diapause hormone has 24 amino acid residues and a molecular weight of 2645. The synthesized peptide with a carboxyl terminal as amide form exhibited the same behavior on HPLC and similar activity as the natural hormone.

• 日本学士院の論文

著者

• Yamashita Okitsugu

  Laboratory Of Sericultural Science

• Imai Kunio

  Laboratory Of Bioorganic Chemistry Graduate School Of Bioresources Mie University

• Goto Toshio

  Laboratory Of Organic Chemistry Faculty Of Agriculture Nagoya University

• Isobe Minoru

  Laboratory Of Organic Chemistry Bioagricultural Sciences Nagoya University

• NAKAZAWA Yoshitaka

  Laboratory of Chemurgy, Faculty of Bioresources, Mie University

• KOMIYA Takashi

  Laboratory of Chemurgy, Faculty of Bioresources, Mie University

• SAKAKIBARA Kiyoshi

  Laboratory of Sericultural Science, School of Agriculture, Nagoya University

• KOGA Kazushi

  Laboratory of Organic Chemistry, School of Agriculture, Nagoya University

• YAGINUMA Toshinobu

  Laboratory of Sericultural Science, School of Agriculture, Nagoya University

• KONNO Takamichi

  Laboratory of Chemurgy, Faculty of Bioresources, Mie University

• HASEGAWA Kinsaku

  Laboratory of Sericultural Science, School of Agriculture, Nagoya University

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