Changing profiles of mRNA levels of Cdc2 and a novel Cdc2-related kinase (Bcdrk) in relation to ovarian development and embryonic diapause of Bombyx mori

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概要

• 論文の詳細を見る

• To reveal the regulatory mechanism of G_2-arrest of cell division in embryos at the diapause stage of the silkworm, Bombyx mori, the profiles for mRNA levels of a Bombyx homologue of Cdc2 and a novel Bombyx Cdc2-related kinase (Bcdrk) were examined during ovarian development, early embryogenesis and diapause stage, using a reverse transcription/polymerase chain reaction system. Although levels of mRNAs for Cdc2 and Bcdrk were higher in paired ovaries during the early pupal stage, the levels decreased at the middle stage. Both mRNAs became abundant in oocytes towafd the maturation. From oviposition up to the stage just before cellular blastoderm, high levels of both mRNAs were maintained, and thereafter the levels declined. In diapausing eggs kept at 25℃, mRNA levels remained lower, whilst in diapause eggs exposed to 5℃ from 2 days after oviposition, in order to break diapause, relatively higher levels of mRNAs were found. In eggs treated with HCl to avert the entry into diapause, mRNA levels per egg for Cdc2 and Bcdrk increased 2 days after treatment, although these increases were not observed in terms of mRNA levels per actin mRNA. These results were discussed in relation to the activities of nuclear/cellular division in ovaries and eggs. In addition, Bcdrk was suggested to play a role similar to that in Bombyx Cdc2 kinase, because the changing profile for levels of Bcdrk mRNA resembled that for cdc2 mRNA.

• 日本応用動物昆虫学会の論文
• 1998-11-25

著者

• 新美 輝幸

  名古屋大学大学院生命農学研究科

• YAMASHITA Okitsugu

  Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University

• Yamashita Okitsugu

  Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University

• Yamashita Okitsugu

  Laboratory Of Sericultural Science

• Yamashita Okitsugu

  Laboratory Of Sericultural Science Faculty Of Agriculture Nagoya University:(please Address)reprint

• 新美 輝幸

  名古屋大・院生命農・資源昆虫学

• 新美 輝幸

  Graduate School Of Bioagricultural Sciences Nagoya University

• Iwasaki Hideo

  Mechanical Systems Laboratory Toshiba Corporation

• Takahashi M

  Kubota Corp Ibaraki Jpn

• Niimi T

  Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University

• IWASAKI Hideo

  Graduate School of Human Informatics

• NIIMI Teruyuki

  Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya U

• YAGINUMA Toshinobu

  Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya U

• Iwasaki H

  Mechanical Systems Laboratory Toshiba Corporation

• YAGINUMA Toshinobu

  Graduate School of Bioagricultural Sciences, Nagoya University

• Niimi Teruyuki

  Nagoya Univ. Nagoya Jpn

• Iwasaki H

  Institute For Materials Research Tohoku University

• YAGINUMA Toshinobu

  Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University

• Takahashi Michiyoshi

  National Institute of Sericultural and Entomological Science

• Yaginuma Toshinobu

  Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University

• YAGINUMA Toshinobu

  Laboratory of Sericultural Science, School of Agriculture, Nagoya University

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