Establishment of a Sandwich ELISA System to Detect Diapause Hormone, and Developmental Profile of Hormone Levels in Egg and Subesophageal Ganglion of the Silkworm, Bombyx mori(Neurobiology)

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概要

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• In the silkworm Bombyx mori, diapause hormone (DH) is produced in the female subesophageal ganglion (SG) and induces embryonic diapause by targeting developing ovaries. DH is processed from a precursor protein consisting of DH, pheromone biosynthesis activating neuropeptide (PBAN) and three other neuropeptides (SGNPs). Because these five neuropeptides share a common sequence, FXPRLamide, at the C-terminus, a direct and specific assay for DH itself is required in order to understand the profile of concentration changes. In this study, we produced a mouse monoclonal antibody (anti-DH[N] mAb) against the N-terminal region of DH and developed a sandwich enzyme-linked immunosorbent assay using the anti-DH[N] mAb and a rabbit polyclonal antibody against the C-terminus of DH. This procedure enabled us to specifically quantify the DH molecule at femtomolar levels (equivalent to 1/10 of SG). We then plotted DH levels in eggs and SGs during embryonic and post-embryonic development. DH was present in late-stage embryos that had been destined for the production of both diapause and nondiapause eggs. DH levels in SG gradually increased in both types during larval development and peaked at the early pupal stage. At the middle pupal stage, DH levels in SG and SG-brain complex decreased markedly in the diapause-egg producing type, thus indicating active release of DH into the hemolymph. From 5th instar larva to adult, no sexual differences in DH levels were observed in SGs or SG-brain complexes from diapause and nondiapause egg-producing types.

• 社団法人日本動物学会の論文
• 2005-02-25

著者

• 新美 輝幸

  名古屋大学大学院生命農学研究科

• SHIOMI Kunihiro

  Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu Un

• YAMASHITA Okitsugu

  Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University

• Shiomi Kunihiro

  Laboratory Of Silkworm Genetics And Pathology Faculty Of Textile Science And Technology Shinshu Univ

• Shiomi Kunihiro

  Shinshu Univ. Nagano Jpn

• Niimi Takahiro

  Department Of Life Science Graduate School Of Science Himeji Institute Of Technology

• Yamashita Okitsugu

  Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University

• Yamashita Okitsugu

  Laboratory Of Sericultural Science

• Yamashita Okitsugu

  Laboratory Of Sericultural Science Faculty Of Agriculture Nagoya University:(please Address)reprint

• 新美 輝幸

  名古屋大・院生命農・資源昆虫学

• 新美 輝幸

  Graduate School Of Bioagricultural Sciences Nagoya University

• Imai Kunio

  Mie Univ. Tsu Jpn

• Imai Kunio

  Laboratory Of Organic Chemistry Faculty Of Agriculture Nagoya University

• Imai Kunio

  Faculty Of Bioresources Mie University

• Niimi T

  Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University

• Niimi Teruyuki

  Graduate School Of Bioagricultural Sciences Nagoya University

• NIIMI Teruyuki

  Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya U

• YAGINUMA Toshinobu

  Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya U

• KITAGAWA Norio

  Graduate School of Bioagricultural Sciences, Nagoya University

• YAGINUMA Toshinobu

  Graduate School of Bioagricultural Sciences, Nagoya University

• Kitagawa Norio

  Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya U

• Niimi Teruyuki

  Nagoya Univ. Nagoya Jpn

• Shiomi Kunihiro

  Laboratory Of Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya Uni

• YAGINUMA Toshinobu

  Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University

• Kitagawa Norio

  Laboratory Of Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya Uni

• Yaginuma Toshinobu

  Sericulture And Entomoresources Graduate School Of Bioagricultural Sciences Nagoya University

• Imai Kunio

  Faculty Of Bio-resources Mie University

• IMAI Kunio

  Faculties of Bioresources, Mie University

• IMAI Kunio

  Faculties of Bioresource, Mie University

• YAGINUMA Toshinobu

  Laboratory of Sericultural Science, School of Agriculture, Nagoya University

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