New Analysis Method for Protein Phosphatase Type 2A Inhibitors Using the Firefly Bioluminescence System

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概要

• 論文の詳細を見る

• A new analysis method for protein phosphatase type 2A inhibitors was established that uses the firefly bioluminescence system for detection. Thus, firefly luciferin phosphate was used as a substrate, and the liberated free luciferin was determined from the amount of light emitted from the immobilized luciferase. This method was successfully used to determine the activities of known inhibitors, i.e., okadaic acid, calyculin A, microcystin-LR and tautomycin using less than 10 pmol of a sample.

• 社団法人日本農芸化学会の論文
• 1995-12-23

著者

• ISOBE Minoru

  Laboratory of Organic Chemistry, School of Bioagricultural Sciences, Nagoya University

• Isobe M

  Nagoya Univ. Nagoya Jpn

• Isobe Minoru

  Laboratory Of Organic Chemistry Faculty Of Agriculture Nagoya University

• Sugiyama Y

  Kyoto Prefectural Univ. Coll. Agriculture Kyoto Jpn

• Sugiyama Yoshio

  Integrated Technology Laboratories Takeda Chemical Industries Ltd.

• Takai Akira

  Department Of Physiology School Of Medicine Nagoya University

• SUGIYAMA Yukiteru

  Laboratory of Organic Chemistry, School of Agricultural Sciences, Nagoya University

• OHTANI Ikuko

  Laboratory of Organic Chemistry, School of Agricultural Sciences, Nagoya University

• Ito Takeharu

  Laboratory of Organic Chemistry, School of Agricultural Sciences, Nagoya University

• Toya Yoshiaki

  Laboratory of Organic Chemistry, School of Agricultural Sciences, Nagoya University

• Nishigohri Yoshiko

  Laboratory of Organic Chemistry, School of Agricultural Sciences, Nagoya University

• Ito Takeharu

  Laboratory Of Organic Chemistry School Of Agricultural Sciences Nagoya University

• Ohtani I

  Laboratory Of Organic Chemistry School Of Agricultural Sciences Nagoya University

• Ohtani Ikuko

  Laboratory Of Organic Chemistry School Of Agricultural Sciences Nagoya University

• Toya Yoshiaki

  Laboratory Of Organic Chemistry School Of Agricultural Sciences Nagoya University

• Toya Yoshiaki

  Laboratory Of Organic Chemistry Aichi University Of Education

• Takai Akira

  Department Of Physiology Medical School Nagoya University

• Nishigohri Yoshiko

  Laboratory Of Organic Chemistry School Of Agricultural Sciences Nagoya University

• Isobe Minoru

  Laboratory Of Organic Chemistry Bioagricultural Sciences Nagoya University

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