Production of Pyridoxal Phosphate by a Mutant Strain of Schizosaccharomyces pombe

概要

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Conditions for extracellular production of vitamin B_6 compounds (B_6), especially pyridoxal 5'-phosphate (PLP) by Schizosaccharomyces pombe leul strain were examined. The productivity was dependent on concentration of L-leucine in the culture medium : 30 mg/1 gave the highest concentrations of total B_6 and PLP. The viable cells harvested at different growth phases showed different productivity : middle and late exponential phase cells showed the highest productivity of total B_6 and PLP, respectively. D-Glucose (1%, w/v) among other sugars gave the best productivity. Supplementation of air and ammonium sulfate significantly increased extracellular production of PLP. Superoxide anion producers, menadione and plumbagin, and H_2O_2 increased the productivity of PLP. Cycloheximide inhibited the increase of PLP by the oxidative stress and, in contrast, increased pyridoxine.
社団法人日本農芸化学会の論文
2001-08-23