Purification, Molecular Cloning, and Characterization of Pyridoxine 4-Oxidase from Microbacterium luteolum
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概要
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Pyridoxine 4-oxidase (EC 1. 1. 3. 12, PN 4-oxidase), which catalyzes the oxidation of PN by oxygen or other hydrogen acceptors to form pyridoxal and hydrogen peroxide or reduced forms of the acceptors, respectively, was purified for the first time to homogeneity from Microbacterium luteolum YK-1 (=Aureobacterium luteolum YK-1). The purified enzyme required FAD for its catalytic activity and stability. The enzyme was a monomeric protein with the subunit molecular mass of 53,000 ± 1,000 Da. PN was the only substrate as the hydrogen donor. Oxygen, 2,6-dichloroindophenol, and vitamin K_3 were good substrates as the hydrogen acceptor. The gene (pno) encoding PN 4-oxidase was cloned. The gene encodes a protein of 507 amino acid residues corresponding to the molecular mass of the subunit. PN 4-oxidase was expressed in Escherichia coli and found to have the same properties as the native enzyme from M. luteolum YK-1. Comparisons of primary and secondary structures with other proteins showed that the enzyme belongs to the GMC oxidoreductase family. M. luteolum YK-1 has four plasmids. The pno gene was found on a chromosomal DNA. Search for genes similar in sequence in other organisms suggested that a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti, which harbors two plasmids, has a PN degradation pathway I in chromosomal DNA.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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OHNISHI Kouhei
Research Institute of Molecular Genetics, Kochi University
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YAGI Toshiharu
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Ohnishi Kouhei
Research Institute Of Molecular Genetics Kochi University
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Yagi Toshiharu
Department Of Agricultural Chemistry Kochi University
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Yagi T
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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KANEDA Yasuo
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Kaneda Yasuo
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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