Molecular Cloning, Identification and Characterization of 2-Methyl-3-Hydroxypyridine-5-Carboxylic-Acid-Dioxygenase-Coding Gene from the Nitrogen-Fixing Symbiotic Bacterium Mesorhizobium loti(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• The gene (mlr6788) of a nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099 has been identified as a gene coding for 2-methyl-3-hydroxypyridine-5-carboxylic acid dioxygenase (MHPCO), the seventh enzyme in degradation pathway I for pyridoxine, a free form of vitamin B_6. The gene was cloned and overexpressed in Escherichia coli cells co-transformed with chaperonin genes. The homogeneous recombinant enzyme showed similar enzymatic properties to the enzyme from Pseudomonas sp. MA-1. MHPCO was essential for the assimilation ofpyridoxine in M. loti, but not for its growth in a nutrient-rich medium. From the infection experiment of a symbiotic plant Lotus japonicm with an M. loti mlr6788 gene disruptant, MHPCO was demonstrated to be dispensable for at least nodule formation on roots of seedlings in symbiosis.

• 社団法人日本生物工学会の論文
• 2006-12-25

著者

• Yagi Toshiharu

  高知大・農学部門

• YUAN Baiqiang

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• YOKOCHI Nana

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• YOSHIKANE Yu

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• OHNISHI Kouhei

  Research Institute of Molecular Genetics, Kochi University

• YAGI Toshiharu

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Yokochi Nana

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• Ohnishi Kouhei

  Research Institute Of Molecular Genetics Kochi University

• Yagi Toshiharu

  大阪医科大学 生化学

• Yagi Toshiharu

  Department Of Agricultural Chemistry Kochi University

• Yoshikane Yu

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• Yuan Baiqiang

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• Ohnishi Kouhei

  Kochi Univ. Kochi Jpn

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