Synthesis of 4-Pyridoxolactone from Pyridoxine Using a Combination of Transformed Escherichia coli Cells(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

概要

論文の詳細を見る
We developed a simple and efficient synthesis for 4-pyridoxolactone starting with pyridoxine and using a whole-cell biotransformation by two transformed Escherichia coli cell types. One set of transformed cells expressed pyridoxine 4-oxidase, catalase, and chaperonin, while the second set expressed pyridoxal 4-dehydrogenase. With this combination of cells, pyridoxine was first oxidized to pyridoxal, which was then dehydrogenated to 4-pyridoxolactone by pyridoxine 4-oxidase and pyridoxal 4-dehydrogenase, respectively. In a reaction mixture containing the two transformed cell types, 10mM of pyridoxine was completely converted into 4-pyridoxolactone at 30℃ in 24h. When starting with 80mM of pyridoxine, it was necessary to add 0.5mM or more of NAD^+ to complete the reaction.
社団法人日本生物工学会の論文
2008-11-25