Determination of Individual Vitamin B6 Compounds Based on Enzymatic Conversion to 4-Pyridoxolactone
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概要
- 論文の詳細を見る
A determination method for individual natural vitamin B_6 compounds was developed. The vitamin Be compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-β-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-β-glucoside were not separately determined here. Three food samples were analyzed by this method.
- 日本ビタミン学会の論文
著者
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Yagi Toshiharu
高知大・農学部門
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YOKOCHI Nana
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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YOSHIKANE Yu
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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GE Fei
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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YAGI Toshiharu
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Yokochi Nana
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Yagi Toshiharu
大阪医科大学 生化学
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Yagi Toshiharu
Department Of Agricultural Chemistry Kochi University
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Ge Fei
Kochi Univ. Kochi Jpn
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Yoshikane Yu
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Ge Fei
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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NISHIMURA Saki
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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NAGANO Sayaka
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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CRAI Chan
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Crai Chan
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Nagano Sayaka
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Nishimura Saki
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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