Distribution and Cellular Localization of Aspartate Aminotransferase Isoenzymes in Rice

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概要

• 論文の詳細を見る

• Aspartate aminotransferase (EC 2.6.1.1, AAT) occurs as two forms in rice bran (from Oryza sativa cv. Koganemasari), AAT-1 and AAT-2. Immunotitrations with antisera against the AAT isoenzymes indicated an immunological distinction between AAT-1 and AAT-2. Sequential titration of total AAT activity showed that AAT-1 and AAT-2 comparised 82% and 18% of the total activity in the crude extract of rice bran, respectively. AAT-1 and AAT-2 comprised 45% and 55% of the total AAT activity in the crude extract of top tissues of the rice plant. AAT-1 was mainly in the cytosolic fraction of the rice plant cells. On the other hand, AAT-2 was the major isoenzyme of AAT in mitochondria and peroxisomes. Minute activity of AAT-1 was found in the membrane fractions of the mitochondria and peroxisomes and was solubilized with Triton X-100.

• 社団法人日本農芸化学会の論文
• 1993-12-23

著者

• YAGI Toshiharu

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Yagi T

  Department Of Bioresources Science Kochi University

• Yagi Toshiharu

  Department Of Agricultural Chemistry Kochi University

• Yagi T

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• YAMAMOTO Shinpei

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Yamamoto Shinpei

  Department Of Agricultural Chemistry Faculty Of Agriculture Kochi University

• Yamamoto Shinpei

  Department Of Agricultural Chemistry Kochi University

• Yamamoto Shinpei

  Department Of Bioresources Science Kochi University

• Masaki Kazunari

  Department of Bioresources Science, Kochi University

• Masaki Kazunari

  Department Of Bioresources Science Kochi University

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