An Enzymatic Fluorometric Assay for Pyridoxal with High Specificity and Sensitivity
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概要
- 論文の詳細を見る
An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol : the calibration curve of pyridoxal showed high linearity (r = 0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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YAGI Toshiharu
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Yagi Toshiharu
Department Of Agricultural Chemistry Kochi University
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TRONGPANICH Yanee
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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MITO Mitsunori
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Mito Mitsunori
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Trongpanich Yanee
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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