Gene Identification and Characterization of the Pyridoxine Degradative Enzyme α-(N-Acetylaminomethylene)succinic Acid Amidohydrolase from Mesorhizobium loti MAFF303099
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概要
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We have found for the first time that a chromosomal gene, mlr6787, in Mesorhizobium loti encodes the pyridoxine degradative enzyme α-(N-acetylaminomethylene)succinic acid (AAMS) amidohydrolase. The recombinant enzyme expressed in Escherichia coli cells was homogeneously purified and characterized. The enzyme consisted of two subunits each with a molecular mass of 34,000±1,000 Da, and exhibited Km and kcat values of 53.7±6 μM and 307.3±12 min^<-1>, respectively. The enzyme required no cofactor or metal ion. The primary structure of AAMS amidohydrolase was elucidated for the first time here. The primary structure of the enzyme protein showed no significant identity to those of known hydrolase proteins and low homology to those of fluoroacetate dehalogenase (PDB code, 1Y37), haloalkane dehalogenase (1K5P), and aryl esterase (1VA4).
著者
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Yagi Toshiharu
高知大・農学部門
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YUAN Baiqiang
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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YOKOCHI Nana
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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YOSHIKANE Yu
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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OHNISHI Kouhei
Research Institute of Molecular Genetics, Kochi University
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GE Fei
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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YAGI Toshiharu
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Yokochi Nana
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Ohnishi Kouhei
Research Institute Of Molecular Genetics Kochi University
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Yagi Toshiharu
大阪医科大学 生化学
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Yagi Toshiharu
Department Of Agricultural Chemistry Kochi University
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Ge Fei
Kochi Univ. Kochi Jpn
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Yoshikane Yu
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Yuan Baiqiang
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Ohnishi Kouhei
Kochi Univ. Kochi Jpn
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Ge Fei
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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