Simple Affinity Purification Method for Raw Starch-adsorbable and -digesting Amylases with a Raw Starch Column

スポンサーリンク

概要

• 論文の詳細を見る

• A simple purification procedure for raw starch-adsorbable and -digesting amylases (RSAs) was devised. The method depended on an affinity column, which was prepared by mixing raw corn starch and Hyflo Super-Cel. RSAs were specifically adsorbed on the matrix, and eluted with a buffer containing 1% β-cyclodextrin. This column could be used to purify RSAs from Streptomyces thermo-cyaneoviolaceus and a recombinant strain of E. coli.

• 社団法人日本農芸化学会の論文
• 1996-10-23

著者

• YAGI Toshiharu

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Yagi Toshiharu

  Department Of Agricultural Chemistry Kochi University

• YAMAMOTO Shinpei

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Yamamoto Shinpei

  Department Of Agricultural Chemistry Faculty Of Agriculture Kochi University

• Yamamoto Shinpei

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• Mai Hang

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• FURUYOSHI Setsuo

  Research Institute of Molecular Genetics, Kochi University

• Furuyoshi Setsuo

  Research Institute Of Molecular Genetics Kochi University

関連論文

• Gene Identification and Characterization of the Pyridoxine Degradative Enzyme α-(N-Acetylaminomethylene)succinic Acid Amidohydrolase from Mesorhizobium loti MAFF303099
• Purification, Molecular Cloning, and Characterization of Pyridoxine 4-Oxidase from Microbacterium luteolum
• Optimization for β-Mannanase Production of a Psychrophilic Bacteriunm, Flavobacterium sp.
• Mannanase of Psycrophilic Bacteria
• Recombinant Expression, Biochemical Characterization and Stabilization through Proteolysis of an L-Glutamate Oxidase from Streptomyces sp. X-119-6
• Purification and Characterization of Pyridoxal 4-Dehydrogenase from Aureobacterium luteolum
• An Enzymatic Fluorometric Assay for Pyridoxal with High Specificity and Sensitivity
• Purification and characterization of raw-starch-digesting : α-amylase from Streptomyces thermocyanoviolaceus
• Simple Affinity Purification Method for Raw Starch-adsorbable and -digesting Amylases with a Raw Starch Column
• Production of Pyridoxal Phosphate by a Mutant Strain of Schizosaccharomyces pombe
• Efflux System for Pyridoxine in Schizosaccharomyces pombe
• 1-II-6 Production of vitamin B_6 compounds by a mutant strain of Schizosaccharomyces pombe
• Distribution and Cellular Localization of Aspartate Aminotransferase Isoenzymes in Rice
• Purification and Characterization of Aspartate Aminotransferase Isoenzymes from Rice Bran
• Interaction of Pyridoxal 5'-Phosphate Form of Aspartate Aminotransferase with Vitamin B-6 Compounds and Antagonists in Rabbit Erythrocytes
• Gene Identification and Characterization of 5-Formyl-3-Hydroxy-2-Methylpyridine 4-Carboxylic Acid 5-Dehydrogenase, an NAD^+-Dependent Dismutase
• Synthesis of 4-Pyridoxolactone from Pyridoxine Using a Combination of Transformed Escherichia coli Cells(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Gene Identification and Characterization of the Pyridoxine Degradative Enzyme 4-Pyridoxic Acid Dehydrogenase from the Nitrogen-fixing Symbiotic Bacterium Mesorhizobium loti MAFF303099
• Determination of Individual Vitamin B6 Compounds Based on Enzymatic Conversion to 4-Pyridoxolactone
• Molecular Cloning, Identification and Characterization of 2-Methyl-3-Hydroxypyridine-5-Carboxylic-Acid-Dioxygenase-Coding Gene from the Nitrogen-Fixing Symbiotic Bacterium Mesorhizobium loti(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Purification and Properties of Bacillus coagulans Cyclomaltodextrin Glucanotransferase
• Cyclomaltodextrin Glucanotransferase-Producing Moderate Thermophile, Bacillus coagulans
• Characterization of Recombinant YakC of Schizosaccharomyces pombe Showing YakC Defines A New Family of Aldo-keto Reductases
• Active Transport Activities of Free B-6 Vitamers in Various Yeast Strains
• Properties and Mode of Action of β-1,3-Glucanase from Rhizoctonia sp. Studies on Fungal Enzymes Active in Hydrolyzing Yeast Cell Wall (VI).
• Purificaiton and Characterization of a Yeast cell-lytic Enzyme of a Species of Rhizoctonia : Studies on Fungal Enzymes Active in Hedrolysing Yeast Cell Wall (V).
• A Rhizoctonia sp. as a Potent Producer of Yeast Cell-lytic Enzyme, and It's Production by Culture and Properties of the Enzyme Isolated : Studies on Fungal Enzymes Active in Hydrolysing Yeast Cell Wall(IV)
• Disruption of the plr1^+ Gene Encoding Pyridoxal Reductase of Schizosaccharomyces pombe
• Topology of 4-Pyridoxic acid dehydrogenase in transformed Escherichia coli cells
• A Simple Colorimetric Assay for Aspartate Aminotransferase

もっと見る 閉じる

スポンサーリンク