Optimization for β-Mannanase Production of a Psychrophilic Bacteriunm, Flavobacterium sp.

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概要

• 論文の詳細を見る

• We found that a psychrophilic bacterium, Flavobacterium sp., characterized in this study, has a β-mannanase (EC 3.2.1.78) activity in the culture medium. The mannanase activity was the highest in the culture medium, containing 1.0% (w/v) guar gum (as a carbon source), 0.3% (NH_4)_2SO_4 (as a nitrogen source), and 0.06% (w/v) yeast extract, of five-days cultivation at 4℃. No mannanase activity was found in the medium containing a monosaccharide or a disaccharide as a carbon source, although the psychrophile could use them. The enzyme activity was found only when the bacterium was cultured in the medium containing a polysaccharide. The enzyme preparation showed a single activity band on a washed gel of SDSPAGE. The optimal temperature for the enzyme activity was 35℃. When the reaction was done at 10℃, the enzyme showed 25% of the optimal activity. The β-mannanase preparation efficiently hydrolyzed guar gum, locust bean gum, and glucomannan as well as β-mannan.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• YAGI Toshiharu

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Zakaria Mia

  Department Of Bioresources Science Kochi University

• Mia Md

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• Yagi T

  Kochi Univ. Kochi Jpn

• Yagi Toshiharu

  Department Of Agricultural Chemistry Kochi University

• ASHIUCHI Makoto

  Research Institute of Molecular Genetics, Kochi University

• YAMAMOTO Shinpei

  Department of Bioresources Science, Faculty of Agriculture, Kochi University

• Ashiuchi Makoto

  Research Institute Of Molecular Genetics Kochi University

• Yamamoto Shinpei

  Department Of Agricultural Chemistry Faculty Of Agriculture Kochi University

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