Estrogen Stabilization of the mRNA of Chicken Ovalbumin Gene in the Cultured Organ from Estrogen Induced-chick Oviduct
スポンサーリンク
概要
- 論文の詳細を見る
Though estrogen prolongs the half-life of chicken ovalbumin (OVA) mRNA in vivo, little has been described about the molecular mechanism by which estrogen stabilizes the OVA mRNA. As a first trial to discover this molecular mechanism, the organ culture of estrogen-induced chick oviduct was introduced to know whether estrogen could stabilize OVA mRNA in vitro, as observed in vivo. In this culture system, the expression of egg white genes were induced and maintained by estrogen. Moreover, using a pulse-chase labeling method, we showed that estrogen stabilized the OVA mRNA, and that the half-lives of the OVA mRNA in the presence and the absence of estrogen were almost identical with those calculated from in vivo study. Thus, from this study, we concluded that this culture system had a faithful feature in egg white gene expression for the response to estrogen, and it will be useful to investigate the mechanism of estrogen-induced stabilization upon the OVA mRNA.
- 社団法人日本農芸化学会の論文
- 1994-02-23
著者
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NINOMIYA Yuichi
Department of Cardiovascular, Respiratory & Metabolic Medicine, Graduate School of Medicine, Kagoshi
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MASUSHIGE Shoichi
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
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HASEGAWA Tadao
Department of Molecular Bacteriology, Nagoya University Graduate School of Medicine
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Kato Shuji
Tobacco Science Research Laboratory Japan Tobacco Inc.
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Hasegawa Toru
Institute For Fermentation Osaka
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Ninomiya Yuichi
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
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KATO Shigeaki
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
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Masushige Shoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
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Kato Shigeaki
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
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Kato S
Institute For Chemical Research Kyoto University
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Hasegawa T
Yokohama City Univ. Yokohama Jpn
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Hasegawa Tadao
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
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Masushige S
Tokyo Univ. Agriculture Tokyo Jpn
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ARAO Yukitomo
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
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MIYATAKE Naoto
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
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Arao Yukitomo
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
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Miyatake Naoto
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
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Kato S
Tobacco Science Research Laboratory Japan Tobacco Inc.
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