Bacterially Expressed Rat Retinol-binding Protein is Functional for Retinol and Transthyretin Bindings
スポンサーリンク
概要
- 論文の詳細を見る
Retinol-binding protein (RBP) was expressed in Escherichia coil using the cDNA for rat RBP, and characterized. The expressed RBP was fused to maltose-binding protein (MBP) at the N-terminal end (MBP-RBP), and MBP was enzymatically removed from the MBP-RBP with proteinase factor Xa. The binding of retinol and transthyretin (TTR) to the recombinant RBP was monitored by means of gel filtration. The recombinant RBP specifically bound to retinol with an affinity similar to that of purified RBP from rat serum. Furthermore, the retinol-bound recombinant RBP formed hetero-complexes with TTR similar to RBP. Thus, the results showed that the recombinant RBP expressed in E. codi is as functional as serum RBP in terms of retinol and TTR bindings.
- 財団法人 学会誌刊行センターの論文
著者
-
KATO Shigeaki
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
-
Masushige Shoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
-
Kato Shigeaki
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
-
Mano H
Department Of Physics Gakushuin University
-
Mano Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo University Of Agriculture
-
Yamamoto Yuji
Department Of Cardiovascular Medicine University Of Tokyo Graduate School Of Medicine
-
YOSHIZAWA Tatsuya
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
-
Yamamoto Yuji
Department Of Applied Biology And Chemistry Tokyo University Of Agriculture
-
MANO Hiroshi
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
-
YAMAMOTO Yuji
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture
関連論文
- Lower Limb Valgus Alignment Compared with Two-and Three-Dimensional Motion Analysis during a Drop Jump Test
- Aqueous Extract of Kotahla Himbutu (Salacia reticulata) Stems Promotes Oxygen Comsumption and Supresses Body Fat Accumulation in Mice
- The Administration of Retinoic Acid Down-Regulates cAMP-Responsive Element Modulator (CREM) mRNA in Vitamin A-Deficient Testes
- Dietary Fiber Suppresses Elevations of Uric Acid and Allantoin in Serum and Urine Induced by Dietary RNA and Increases Its Excretion to Feces in Rats
- Metastatic Osteosarcoma in the Jejunum with Intussusception : Report of a Case
- Clinical Manifestations due to a Point Mutation of the Mitochondrial tRNA^ Gene in Five Families with Diabetes Mellitus
- Significance of Plasma Brain Natriuretic Peptide in Patients with Reversible Left Ventricular Dysfunction takotsubo Cardiomyopathy
- Chronic Reduction in Dietary Tryptophan Leads to Changes in the Emotional Response to Stress in Mice
- Long-Term Outcome After Hernia Repair with the Prolene Hernia System
- Tumor Necrosis Factor-α Enhances Angiotensin II-Induced Reseactive Oxygen Species Production in Vascular Myocytes (Atherosclerosis, Basic 7 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-014 Adremedullin augments collateral development and mobilization of endothelial progenitor cells (EPCs) in response to acute ischemia(Angiogenesis and Regeneration Medicine in Cardiovascular Medicine (M) : FRS3)(Featured Research Session (English))
- Possible Roles of CTGF/Hcs24 in the Initiation and Development of Ossification of the Posterior Longitudinal Ligament
- Stabilized Self-Sustained Sequence Replication against Evolutionary Instability
- Identification of Cytoplasmic and Nuclear Low-Molecular-Weight Heat-Shock Proteins in Tomato Fruit
- Cloning, Nucleotide Sequence, and Disruption of Streptococcus mutans Glutathione Reductase Gene (gor)
- Glucosamine Regulates Differentiation of a Chondrogenic Cell Line, ATDC5(Molecular and Cell Biology)
- Proportion of Murine Cytotoxic T Cells is Increased by High Molecular-Weight Fucoidan Extracted from Okinawa mozuku (Cladosiphon okamuranus)
- Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction
- Significance of volume-reduction surgery for far-advanced gastric cancer during treatment with novel anticancer agents
- Fatigue and Tribological Properties of Materials for Hydrogen Service
- Immunochemical and Immunohistochemical Studies on Distribution of Elastin Fibres in Human Atherosclerotic Lesions using a Polyclonal Antibody to Elastin-derived Hexapeptide Repeat
- Steroid Hormones Differentially Induce Transcription of the Chicken Ovalbumin Gene, But Stabilize the mRNA with the Same Half-Life
- Steroid Hormone-induced Expression of the Chicken Ovalbumin Gene and the Levels of Nuclear Steroid Hormone Receptors in Chick Oviduct
- Estrogen Stabilization of the mRNA of Chicken Ovalbumin Gene in the Cultured Organ from Estrogen Induced-chick Oviduct
- Metachronous Intraductal Papillary Mucinous Neoplasm with Carcinoma In Situ of the Pancreas Arising Within a Short Interval : Report of a Case
- Effects of Various Bone Metabolism-related Factors on a Pure Population of Mature Osteoclasts
- Precision Grinding of Micro Array Lens Molding Die with 4-axes Controlled Micro Wheel(Ultra-precision machining)
- Cleavage of Bovine Mitochondrial ATPase Inhibitor with Endopeptidases, and Binding of the Resulting Peptides to the Interface between the α-and β-Subunits of F_1ATPase^1
- Study of Critical Phenomena by Spin-Cluster Approximation Series:Ising Spin System
- Sudden unexpected death due to rupture of the stomach
- Double-injection method for sequentially measuring cerebral blood flow with N-isopropyl-(^I)p-iodoamphetamine
- Safety Evaluation of the Aqueous Extract Kothala Himbutu (Salacia reticulata) Stem in the Hepatic Gene Expression Profile of Normal Mice Using DNA Microarrays
- Long-term survival after multimodal therapy in a patient demonstrating intrahepatic cholangiocarcinoma with hilar invasion and intrahepatic metastases
- Hamartin-Hsp70 Interaction Is Necessary for Akt-Dependent Tuberin Phosphorylation during Heat Shock
- Calculation of Local Environment Effects in Ferromagnetic Transition-Metal Alloys with Atomic Short-Range Order : Method and Application to Fe-V
- Identification of a Nuclear Retinoic Acid-binding Activity Representative of Endogeneous Retinoic Acid Receptors in the Nuclei of Rat Liver and Testis
- Heat Capacity of Two Dimensional Heisenberg Antiferromagnet with s=5/2
- Dietary Fiber Suppresses Elevations of Uric Acid and Allantoin in Serum and Urine Induced by Dietary RNA and Increases Its Excretion to Feces in Rats
- A Theory of Local Environment Effect on the Electronic Structure in Disordered Alloys
- Bacterially Expressed Rat Retinol-binding Protein is Functional for Retinol and Transthyretin Bindings
- Possible Phase Diagrams of Systems with Competing Anisotropies
- Comparison between the Effect of Collagen Tripeptide and Sodium Hyaluronan to Prevent Knee Osteoarthritis : A Preliminary in vivo Study
- Discrepancy between Spin-Wave Dispersion and Temperature Dependence of Magnetization in Amorphous Ferromagnets
- A new method for ABO genotyping using a multiplex single-base primer extension reaction and its application to forensic casework samples
- A New Cluster Approximation to the Diluted Ising and Heisenberg Ferromagnets
- Pair-Spin Theory of a Random Mixture with Competing Ising and XY Anisotropies
- Gene Expression of Cellular Retinol-Binding Protein I (CRBP I) is Affected by Dietary Proteins in the Rat Liver
- Role of Second-Neighbor Interaction in Diluted Ferromagnetic Systems
- On Scuffing Conditions under Rolling/Sliding Contact : Part 2 In the case of refined 0.45% carbon steel
- Influences of Maternal B12 and Methionine Intake during Gestation and Lactation on Testicular Development of Offspring in Rats
- Long-Term Effect of Imidapril Hydrochloride Compared With Dilazep Hydrochloride Administration on Blood Pressure and Renal Function in Patients With Chronic Glomerulonephritis
- Production of Anthocyanins by Vitis Cells in Suspension Culture
- Evidence for Other Binding Component(s) than Retinoid Receptors Specifically Interacting with a Retinoic Acid Response Element in the Rat Liver.
- Formation and Identification of Anthocyanins ijn Cultured Cells of Vitis sp.
- Purification and Some Properties of Cell-bound Lipase from Saccharomycopsis lipolytica
- High-Efficiency Conversion of Threading Screw Dislocations in 4H-SiC by Solution Growth
- A Base-induced Elimination Reaction of Phenylsulfonylacetates. II. Effects of the Base and the Solvent on the Stereochemistry of Elimination