Production of Various Phosphatidylsaccharides by Phospholipase D from Actinomadura sp. Strain No. 362

概要

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Various phosphatidylsaccharides can be produced in good yields by a one-step transphosphatidylation reaction catalyzed by the purified phospholipase D from Actinomadura sp. strain No. 362. With glucose as the saccharide, the optimal conditions of transphosphatidylation were investigated. Several solvents such as diethylether or tert-butanol and several salts such as NaCl or CaC1_2 were effective for phosphatidylglucose production. The optimal concentration of glucose for phosphatidylglucose production was 0.8 part of the water. With optimal conditions, the conversion rate of egg lecithin to phosphatidylglucose reached about 85%. With this condition of phosphatidylglucose production, dipalmitoylphosphatidylcholine, dimyristoylphosphatidylcholine, soya lecithin, phosphatidylethanolamine, phosphatidylglycerol, dihexadecylphosphatidylcholine, and sphingomyelin were found to be good substrates. Pentoses, hexoses, and heptoses with primary alcohol groups were also found to be good acceptors of transphosphatidylation.
社団法人日本農芸化学会の論文
1993-08-23