Production and Properties of Alkaline Lipase from Alcaligenes sp. Strain No. 679
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概要
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For the production of extracellular lipase by Alcaligenes species No. 679, NaNO3, polyoxy-ethylene alkyl ether, Fe + +, sodium citrate and fructose were found to be effective. The enzyme was prepared by acetone precipitation from the filtrate of the culture broth of this strain. The enzyme was most active at pH 9.0 and 50°C, while 35% of its activity was lost on heat treatment at 60°C for l0min. Sodium salts of bile acids stimulated the enzyme activity. This lipase could hydrolyse natural fats and oils as well as olive oil. During the hydrolysis of olive oil, monoglyceride was found to accumulate up to 70 mol percent. This lipase possesses special properties similar to those of pancreatic lipase as shown in the comparative experiments.
- 社団法人 日本農芸化学会の論文
著者
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Machida Haruo
Tokyo Research Laboratory, Meito Sangyo Co., Ltd.
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Iwasaki Shinjiro
Tokyo Research Laboratory, Meito Sangyo Co., Ltd.
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Machida Haruo
Tokyo Research Laboratory Meito Sangyo Co. Ltd.
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Kokusho Yoshitaka
Tokyo Research Laboratory Meito Sangyo Co. Ltd.
関連論文
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- Isolation and Identification of Phospholipase D Producing Actinomycetes
- Studies on Alkaline Lipase: Isolation and Identification of Lipase Producing Microorganisms
- Production and Properties of Alkaline Lipase from Alcaligenes sp. Strain No. 679