蛍光抗体法による Nephrotoxin 腎炎の研究 : VII. FITC をラベルした Nephrotoxic Serum による正常腎の蛍光染色所見

概要

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We have previously demonstrated that each antiserum against the tryptic digested ultrasupernatant substance of rat kidney cortex, glomerulus, glomerular basement membrane (GBM), lung, aorta, heart, muscle and liver could produce typical proliferative glomerulonephritis and fluorescein labeled these nephrotoxic serum fixed only in GBM, when injected into normal rats. Present studies were undertaken to investigate the in vitro reaction between these potent nephrotoxic serum and normal kidney by means of direct immunofluorescent method. Staining of normal rat kidney with fluorescein labeled nephrotoxic serum has demonstrated that all these antisera react with GBM, tubular basement membrane (TBM) including Bowman's capsule and media of blood vessels. In the antiserum against kidney cortex and liver, specific fluorescence was found in tubular cytoplasms as well as in GBM, TBM, and blood vessels. This result indicates that an antigenic substance common with tubularcytoplasms is present in liver, although this antigen seems to be not related to nephrotoxic serum antigen. It is already evident that GBM and blood vessels contain nephrotoxic serum antigen. However, further investigation is necessary to clarify whether this specific antigen is also contained in TBM or the fluorescence found in TBM is "non nephrotoxic serum antigen" which widely distributes in basement membrane systems.
日本アレルギー学会の論文
1969-10-30