螢光抗体法によるNephrotoxin腎炎の研究 : とくにラベル抗体静注法による検討

概要

論文の詳細を見る
Since the work of Orgega & Mellors the indirect method of fluoresceint antibody technique has been exclusively used for the demonstration of nephrotoxin in vivo. As far as the immun reaction in vivo is concerned, however, the analysis using the intravenousinjection of fluorescen labeled nephrotoxin should be the first choice. After preparing highly specific F.I.T.C. labeled nephrotoxic rabbit gamma-glubulin with F/P molar ratio fast equal to 1.0., we perofrmed several experiments on the nephrotoxic nephritis injecting these antibodies intravenously. The most remarkable finding by this method is that specific fluorescence in glomeruli localizes only in porper basement membrane compared to the indirect method (staining with fluorescen anti-rabbit gamma-globulin), by which specific fiuorescein are recognized not only in basement membrane proper, but also in epithlial and endothelial structures. On the other hand, an in vitro staining of normal rat kidney with this labeled nephrotoxic antibodies shows that specific fluorescence distributes in Bowman's capsules, tubular cytoplasm, its basement membrane and small blood vessels as well as glomerular capillary loops. It is a very striking fact that the same antibodies react in quite different way in vivo and in vitro. These data suggest that the so-called renal tissue antigenicity and that of inducing nephrotoxic nephritis in vivo is an essentially different problem. Furthermore, following experiments were performed. (I) The application of our method to the study of purification of effective nephrotoxic antigens. (2) The very short term (within 20 minutes) and long term (till 8 weeks) observation of specificfluorescence in gloeruli after injection of labeled nephrotoxin.
日本アレルギー学会の論文

螢光抗体法によるNephrotoxin腎炎の研究 : とくにラベル抗体静注法による検討

スポンサーリンク

概要

著者

関連論文

スポンサーリンク