VM26(epipodophyllotoxin)培養 glioma 細胞に及ぼす効果 : -G_0細胞および動員(recruitment)に関する解析-

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In the synchronized chemoradiotherapy of malignant brain tumors it is important to use a drug having a cell cycle phase specificity on G_2-M phase. Moreover, how to recruit G_0 cells to the proliferating pool during therapy is a problem of therapeutic importance for enhancement of the synchronizing effect. The present study was undertaken to elucidate the pharmacological mechanism of VM26 on cell kinetics.Methods: The rat glial tumor C_6 cell line was cultured for 72 hours initially in the RPMI-1640 medium added with 20% FCS, When the cells were in the log phase, VM26 was added to the medium at concentrations of 0.01,0.1,1,10 and 100 μg/ml and culture was continued for further 24, 48 or 72 hours. After these time intervals specimens were taken for the determination of live cell count and then of the cell cycle phase-distribution by the use of a pulse cytophotometer (ICP 11 ). Histograms of frequency distribution thus obtained were analysed mathematically for the calculation of percentages of G_0+G_1, S and G_2+M phases.Results: VM26 was recognized to have pronounced cytostatic effect even at the lowest concentration of 0.01μg/ml. Pulse cytophotometric analysis also showed that tumor cells became synchronized in G_2-M phase after 24-hour-contact with VM26(1 μg/ml:71%). S phase-block was not observed by the use of VM26. The histogram obtained at 48 hours of contact with the higher concentrations (1,10 and 100 μg/ml) of VM26 showed the persistence of 2CDNA peak,The histogram obtained after 72-hour-contact with the drug at these higher concentrations suggested recruitment of G_0 cells to the proliferating pool.VM26 may thus be expected to have a place in the synchronized chemoradiotherapy of malignant brain tumors.
日本脳神経外科学会の論文
1978-08-15

VM26(epipodophyllotoxin)培養 glioma 細胞に及ぼす効果 : -G_0細胞および動員(recruitment)に関する解析-

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