BCNUによる実験脳腫瘍の細胞動態の変化

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概要

• 論文の詳細を見る

• Kinetic changes induced by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) in 9L rat brain tumor were studied in vitro and in vivo by means of conventional autoradiography and flow cytometry (FCM). In vitro, 5μg/ml BCNU, which resulted in 99% cell kill as measured by the colony forming efficiency assay, had the effects that most cells (more than 90%) were accumulated in G_2 or M phase after one cell division, while cells in early G_1 when BCNU had been given progressed to G_2 and never divided. These effects were also revealed in vivo by using CD Fisher rats bearing 9L brain tumors treated with LD_<10>dose of BCNU which caused 99.9% cell kill.Accumulation of cells in G_2 was also shown by flow cytometric study, but this time showed only 50% accumulation of cells at maximum 54 hrs after BCNU treatment. Rest of cells still remained in 2C peak of tumor even 78 hrs after treatment. Growth fraction of this tumor was 47-55%. This result suggests that these cells in 2C peak of tumor consisted of G_0 cells. In summary, effects of BCNU on 9L rat brain tumor cells in vivo were well correlated with that in vitro so far as cycling cells were concerned. But it should be noticed that G_0 cells could remain there even 78 hrs after BCNU treatment. These data also indicate that in vitro study was useful for estimation of the effects of BCNU treatment in vivo if the doses used for treatment in vivo could expect the same cell kill as that in vitro by colony forming efficiency assay. (Supported in part by CA-13525 and CA-19992 from NCI)

• 日本脳神経外科学会の論文
• 1978-09-15

著者

• 星野 孝夫

  カリフォルニア大学サンフランシスコ校脳神経外科 脳腫瘍研究センター

• 星野 孝夫

  カリフォルニア大学(サンフランシスコ)脳神経外科

• 野村 和弘

  東京大学脳神経外科

• 星野 孝夫

  カリフォルニア大学脳神経外科脳腫瘍研究所

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• ヒト脳腫瘍におけるchloroethylnitrosourea 薬剤耐性の評価法 : Northern blot法によるO^6-methylguanine-DNA methyltransferase mRNA 発現量の定量
• Bromodeoxyuridineを用いたヒト脳腫瘍のgrowth fractionの測定
• VM26(epipodophyllotoxin)培養 glioma 細胞に及ぼす効果 : -G_0細胞および動員(recruitment)に関する解析-
• 抗分裂剤よる脳腫瘍の生長解析
• 放射性thymidineによるラット脳腫瘍の標識率の変動に関する解析
• 脳腫瘍摘出手術が残存腫瘍細胞に及ぼす影響に関する一考察
• BCNUによる実験脳腫瘍の細胞動態の変化
• 脳腫瘍の生長解析 (中枢神経系の悪性腫瘍)
• 脳腫瘍動物モデルと治療法の考え方

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