Enzyme Immunoassay of Calcitonin Gene-Related Peptide-like Immunoreactive Substance in Human Plasma and Saliva
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概要
- 論文の詳細を見る
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a calcitonin generelated peptide-like immunoreactive substance (CGRP-IS) was developed. In competitive reactions, the CGRP-antibody was incubated with CGRP standard (or sample) and β-D-galactosidaselabeled synthetic human αCGRP fragment [residue (8-37)](delayed addition). Free and antibody -bound enzyme haptens were separated by using an anti-rabbit lgG coated immunoplate. Activity of' the enzyme on the plate was fluorometrically determined. The present immunoassay allows the detection of 2.1 to 19.5 fmol/mL of CGRP. Using the proposed EIA, CGRP-ISs in human saliva and plasma were determined. The levels of CGRP-IS in human saliva were about 3.2 fmol/mL, which were much higher than that in human plasma.
- 日本医療薬学会の論文
- 1998-08-10
著者
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TAKEYAMA MASAHARU
Department of Anesthesiology and Critical Care Medicine, Kagoshima University School of Medicine
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Ikawa Kazuro
Department Of Clinical Pharmacotherapy Graduate School Of Biomedical Sciences Hiroshima University
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Takeyama Masaharu
Department Of Anesthesiology And Critical Care Medicine Kagoshima University School Of Medicine
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Naito T
浜松医科大学 医学部附属病院薬剤部
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NAITO Takafumi
Division of Hospital Pharmacy, Hamamatsu University School of Medicine
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Takeyama M
Oita Univ. Hospital
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NAGANO TOSHIAKI
Department of Clinical Pharmacy, Oita Medical University
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Nagano Toshiaki
Division Of Hospital Pharmacy Hamamatsu University School Of Medicine
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Ikawa Kazuro
Department Of Clinical Pharmacotherapy Hiroshima University
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Ikawa Kazuro
Department Of Clinical Pharmacotherapy Division Of Clinical Pharmacotherapeutics Graduate School Of
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