高速液体クロマトグラフィーによる血清中チオペンタール濃度測定法の臨床応用

概要

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It can be presumed that liver function will be influenced by the administration of thiopental in the course of barbiturate therapy. For this reason, we established and tested a new method for the therapeutic monitoring of the concentration of thiopental in human serum. In this method, we employed high-performance liquid chromatography (HPLC) to simplify the pretreatment technique, thereby to shorten the amount of time needed for measurement. As a result, chromatographic data, and the calibration curve (Y=0.249X+0.118, r=0.999) obtained indicated that within-, between-run precision, and recovery rates from the serum are all extremely good. The lowest measurable concentration was found to be 0.1μg/ml (Aufs 0.16). Also, in the cross-reaction tests among drugs mainly used in combination, we did not find any influence on the quantitative analysis of thiopental. We, then measured the serum levels in patients to whom thiopental was administered intravenously. By submitting the results of the t-test, we confirmed a significant rise in the concentration of thiopental in patients with the liver failure, as compared with normal patients(p<0.01). Therefore, we conclude that this assay system can provide measurement as fast as possible, with the added benefit of simplified clinical procedures.
日本医療薬学会の論文
1992-02-20