Adsorption, Activity, and Kinetics of Acid Phosphatase as Influenced by Selected Oxides and Clay Minerals

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概要

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• The effects of 3 oxides (Fe, Al, and Mn oxides) and 3 clay minerals (kaolin, montmorillonite, and allophane) on the adsorption and subsequent kinetic properties of acid phosphatase were compared. The amount of enzyme adsorbed by the oxides and clay minerals followed the order: montmorillonite ≫ kaolin > Mn oxide > Fe oxide > Al oxide ≫ allophane. The adsorption isotherms of the enzyme on the oxides and clay minerals, except for montmorillonite and allophane, fitted the Langmuir equation. The activity of the enzyme immobilized by the inorganic components studied was in the order of allophane > kaolin >Fe oxide >montmorillonite > Al oxide ≒ Mn oxide. Compared to the free enzyme, the V_max, K_m, and V_max / K_m values of the immobilized enzyme decreased, increased, and decreased, respectively. Among the oxides or clay minerals, the higher the ability of the inorganic components to adsorb the enzyme, the lower the value of the V_max / K_m ratio of the immobilized enzyme. These findings suggest that the catalytic efficiency of the enzyme complexes formed is determined by the adsorbability of the inorganic components for the enzyme.

• 社団法人日本土壌肥料学会の論文

著者

• Shindo H

  Yamaguchi Univ. Yamaguchi Jpn

• Shindo Haruo

  Faculty of Agriculture, Yamaguchi University

• Shindo H

  Department Of Electronics Faculty Of Engineering Hiroshima University

• Shindo Haruo

  Faculty Of Agriculture Nagoya University : (present Address) Faculty Of Agriculture Yamaguchi Univer

• Nakahara Osamu

  Graduate School of Agriculture, Hokkaido University

• Watanabe Daisuke

  Faculty of Agriculture, Yamaguchi University

• Onaga Tatsuya

  Faculty of Agriculture, Yamaguchi University

• Urakawa Makiko

  Faculty of Agriculture, Yamaguchi University

• Huang Qiaoyun

  Department of Resources, Environment, and Agrochemistry Huazhong Agricultural University

• Watanabe D

  Department Of Gastroenterology Akita University School Of Medicine

• Nakahara Osamu

  Graduate School Of Agriculture Hokkaido University

• Shindo Haruo

  Faculty Of Agriculture Nagoya University

• Watanabe Daisuke

  The Institute Of Scientific And Industrial Research Osaka University

• Urakawa Makiko

  Faculty Of Agriculture Yamaguchi University

• Onaga Tatsuya

  Faculty Of Agriculture Yamaguchi University

• Huang Qiaoyun

  Department Of Resources Environment And Agrochemistry Huazhong Agricultural University

• WATANABE Daisuke

  Faculty of Agriculture, Miyazaki University

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