Effect of different suture materials on the chronic constriction injury model

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The chronic constriction injury (CCI) model, a standard neuropathic pain model, is induced by tying four 4-0 chromic gut ligatures loosely around the rat sciatic nerve. Chromic gut sutures are necessary to induce this model. The import and sale of medical products and medicine in Japan made from bovine tissue were prohibited in 2000 following the outbreak of bovine spongiform encephelopathy (BSE). Since chromic bovine gut sutures are not now available, we studied if other materials could be used to produce the CCI model. We carried out the procedures described by Bennett et al. on 128 male Sprague-Dawley rats (SLS, Hamamatsu, Japan) by ligating the sciatic nerve with chromic gut, monocryl, coated vicryl, silk, nurolon or ethilon. Data was recorded for the level of the inflammation, for nerve-blood barrier function, and for three behavior tests (the von Frey, acetone, and pin-prick tests). There were no significant differences at any postoperative time interval between any of the groups. Although the sciatic nerve was heavily infiltrated with inflammatory cells for all suture materials, there were no clear differences between the six materials. The injected EBA diffused into the sciatic fiber regardless of the suture material. We found that all suture materials induced local inflammation, nerve-blood barrier failure and neuropathic pain as expected in the CCI model. We concluded that all the examined suture materials could be used in place of chromic gut.
大阪歯科学会の論文

Effect of different suture materials on the chronic constriction injury model

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