Multifunctional Flow-injection Biosensor for the Simultaneous Measurement of Creatinine, Glucose, and Urea
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概要
- 論文の詳細を見る
An amperometric flow-injection biosensor system was developed for the simultaneous measurement of creatinine, glucose, and urea, with a single sample injection and one detector. The principle for the amperometric detection of urea and creatinine was based on coupled reactions of three sequentially aligned enzyme reactors, urease or creatinine deiminase/glutamate dehydrogenase/L-glutamate oxidase. Inclusion of a split point and two confluence points between the injector and detector (oxygen electrode) resulted in a flow-injection system composed of three channels. Triple peak recording was obtained by putting two delay coils of different lengths at the channels involving urease and glucose oxidase. The system gave linear calibrations for creatinine, glucose, and urea in the ranges of 0.2-5, 0.2-10, and 0.5-20 mM, respectively. The assay procedure was simple and one run was completed within 4min. The system was reproducible within 5-8% of relative standard deviation.
- 社団法人日本農芸化学会の論文
- 1993-02-23
著者
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Sonomoto Kenji
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University:
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Sonomoto Kenji
Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin
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Kato Yasuhiko
Department of Anesthesiology, Osaka Dental University
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Ogawa Hiroaki-i.
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Kato Yasuhiko
Department Of Anesthesiology Osaka Dental University
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Rui Chang-Sheng
Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology
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Rui Chang-sheng
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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