回転円磁界がシトクロームP450 2C9の酵素活性に与える影響

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Our previous study revealed that exposures to environmental levels of 60 Hz magnetic fields can significantly change DNA synthesis in cell free systems. These magnetic fields appeared to act mainly at the site of DNA polymerase I and reverse transcriptase, both of which require bivalent cations for full activities. Based on these consistent results, we now attempted to elucidate whether or not exposure to these same type magnetic fields could directly influence metal containing enzyme activity. The purpose of the present study was to determine if exposure to an environmental type circularly polarized 60 Hz magnetic field could alter activities of cytochrome P450 (CYP) 2C9,a heme containing enzyme. Replicate sets of tubes containing reaction mixtures, CYP2C9 and diclofenac were prepared and randomly assigned in equal numbers to temperature controlled plastic boxes located within one of two identical units in the same room with 3.5 meters apart. During triplicate experiments, one of the two units was powered to produce magnetic field exposure at levels of 14μT, 70μT, 140μT, or 280μT. The 4'-hydroxylation of diclofenac is catalyzed by CYP2C9,thus the diclofenac assay is specific for measurement of an activity of CYP2C9. In these studies, CYP2C9 metabolized diclofenac to 4'-hydroxydiclofenc was analyzed by high performance liquid chromatographic methods. Exposure to magnetic fields at 70μT, but not at 14,140 or 280μT, induced decreased amounts of 4'-hydroxydiclofenac with a rate of decrease of 3.3% compared with non exposed controls. These results show that an activity of CYP2C9 was influences by 70μT circularly polarized, sine wave varying 60 Hz magnetic fields. One possible explanation for the results is that the magnetic fields may directly affect heme containing CYP2C9 leading to change of its enzyme activities.
香川県立保健医療大学の論文

回転円磁界がシトクロームP450 2C9の酵素活性に与える影響

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