α-Viniferin Suppresses the Signal Transducer and Activation of Transcription-1 (STAT-1)-Inducible Inflammatory Genes in Interferon-γ-Stimulated Macrophages

スポンサーリンク

概要

• 論文の詳細を見る

• α-Viniferin, an oligostilbene of trimeric resveratrol, has been reported to have anti-inflammatory potential in carrageenin-induced paw edema or adjuvant-induced arthritis in animal models. However, little is known about the molecular basis. In this study, α-viniferin at 3 – 10 μM dose-dependently inhibited interferon (IFN)-γ–induced Ser727 phosphorylation of the signal transducer and activation of transcription-1 (STAT-1), a pivotal transcription factor controlling IFN-γ–targeted genes, in RAW 264.7 macrophages, and also IFN-γ–induced activation of the extracellular signal-regulated kinase (ERK)-1, a protein kinase upstream of the Ser727 phosphorylation of STAT-1. However, α-viniferin, only at a higher concentration of 10 μM, inhibited Janus kinase 2–mediated Tyr701 phosphorylation of STAT-1 in the cells. To understand STAT-1–dependent inflammatory responses, we quantified nitric oxide (NO) or chemokines. α-Viniferin at 3 – 10 μM dose-dependently inhibited IFN-γ–induced production of NO, IFN-γ–inducible protein-10 (IP-10), or the monokine induced by IFN-γ (MIG) in RAW 264.7 cells and also that of NO in primary macrophages-derived from C57BL/6 mice. Furthermore, α-viniferin diminished IFN-γ–induced protein levels of inducible NO synthase (iNOS), attenuated mRNA levels of iNOS, IP-10, or MIG as well as inhibited promoter activity of the iNOS gene. In conclusion, this study proposes an anti-inflammatory mechanism of α-viniferin, down-regulating STAT-1–inducible inflammatory genes via inhibiting ERK-mediated STAT-1 activation in IFN-γ–stimulated macrophages.

• 社団法人 日本薬理学会の論文
• 2010-04-20

著者

• LEE Seung

  College of Pharmacy, Yeungnam University

• Lee Chong-Kil

  College of Pharmacy, Chungbuk National University

• HAN Sang-Bae

  College of Pharmacy, Chungbuk National University

• Han Sang-bae

  College Of Pharmacy Chungbuk National University

• Lee Seung

  College Of Pharmacy And Cbitrc Chungbuk National University

• Kim Youngsoo

  College Of Pharmacy & Research Center For Bioresource And Health Chungbuk National University

• Roh Eunmiri

  College of Pharmacy, Chungbuk National University

• Lee Chong-kil

  College Of Pharmacy Cbitrc Chungbuk National University

• Lee Chong-kil

  College Of Pharmacy Chungbuk National University

• Lee Heun-sik

  College Of Pharmacy Chungbuk National University

• CHUNG Eun

  College of Pharmacy, Chungbuk National University

• KWAK Jeong-Ah

  College of Pharmacy, Chungbuk National University

• Chung Eun

  College Of Pharmacy Chungbuk National University

• Roh Eunmiri

  College Of Pharmacy Chungbuk National University

• Kwak Jeong-ah

  College Of Pharmacy Chungbuk National University

関連論文

• Novel Benzo[d]imidazole-2(3H)-thiones as Potent Inhibitors of the α-Melanocyte Stimulating Hormone Induced Melanogenesis in Melanoma B16 Cells
• Inhibitory Effects of Coumarins from the Stem Barks of Fraxinus rhynchophylla on Adipocyte Differentiation in 3T3-L1 Cells
• Dual Inhibition of Cyclooxygenases-2 and 5-Lipoxygenase by Deoxypodophyllotoxin in Mouse Bone Marrow-Derived Mast Cells(Biochemistry/Molecular Biology)
• The Naturally Occurring Flavolignan, Deoxypodophyllotoxin, Inhibits Lipopolysaccharide-Induced iNOS Expression through the NF-kB Activation in RAW264.7 Macrophage Cells(Highlighted paper selected by Editor-in-chief,Biochemistry)
• One-Pot Synthesis of Simple Alkaloids : 2,3-Polymethylene-4(3H)-quinazolinones, Luotonin A, Tryptanthrin, and Rutaecarpine
• Asterosaponins Isolated from the Starfish Asterias amurensis
• 2-Hydroxycinnamaldehyde Inhibits SW620 Colon Cancer Cell Growth Through AP-1 Inactivation
• Inhibitory Effects of Kurarinol, Kuraridinol, and Trifolirhizin from Sophora flavescens on Tyrosinase and Melanin Synthesis(Pharmacognosy)
• Determination of growth hormone-releasing hexapeptide by reversed-phase high-performance liquid chromatography with electrochemical detection
• FUNCTIONAL HUMAN HEPATOCYTES : ISOLATION FROM SMALL LIVER BIOPSY SAMPLES AND PRIMARY CULTIVATION WITH LIVER-SPECIFIC FUNCTIONS
• Inhibition of Phenotypic and Functional Maturation of Dendritic Cells by Manassantin A
• Low Dose Estrogen Supplementation Reduces Mortality of Mice in Estrogen-Dependent Human Tumor Xenograft Model(Miscellaneous)
• Antiplatelet Effect of NQ12 : a Possible Mechanism Through the Arachidonic Acid Cascade
• Inhibitory Effects of Ethaverine, a Homologue of Papaverine, on Monoamine Oxidase Activity in Mouse Brain
• Inhibitory Effects of Ethaverine, a Homologue of Papaverine, on Dopamine Content in PC12 Cells
• Increase of collagen synthesis by obovatol through stimulation of the TGF-β signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast
• Protective role of Bcl-2 on β-amyloid-induced cell death of differentiated PC12 cells : reduction of NF-κB and p38 MAP kinase activation
• Mushroom Tyrosinase Inhibition Activity of Some Chromones
• Inhibitory Effects of Anti-Inflammatory Drugs on Interleukin-6 Bioactivity
• Protective Mechanism of Glycyrrhizin on Acute Liver Injury Induced by Carbon Tetrachloride in Mice(Pharmacology)
• Gallotannin Isolated from Euphorbia Species, 1,2,6-Tri-O-galloyl-β-D-allose, Decreases Nitric Oxide Production through Inhibition of Nuclear Factor-κ>B and Downstream Inducible Nitric Oxide Synthase Expression in Macrophages
• Spontaneous Ultraweak Photon Emission during the Growth of the Cell Population of Cultured HeLa Cell Line
• Testosterone 5α-Reductase Inhibitors, Menaquinone 7 Produced by a Bacillus and Phenazine Methosulfate
• Anti-inflammatory Benzene Diamine Compound Inhibited Toll-Like Receptor 4-Mediated Inducible Nitric Oxide Synthase Expression and Nuclear Factor-KappaB Activation(Pharmacology)
• Spontaneous Ultra-Weak Photon Emission and Delayed Luminescence during Carbon Tetrachloride-Induced Liver Injury and Repair in Mouse
• Identification of Indoline-2-thione Analogs as Novel Potent Inhibitors of α-Melanocyte Stimulating Hormone Induced Melanogenesis
• α-Viniferin Suppresses the Signal Transducer and Activation of Transcription-1 (STAT-1)-Inducible Inflammatory Genes in Interferon-γ-Stimulated Macrophages
• Synthesis and Biological Properties of Benzo-Annulated Rutaecarpines
• Analysis of Cyclin-Dependent Kinase 2-Regulated Phosphorylation of Stathmin in Etoposide-Induced Apoptotic HeLa Cells by Two-Dimensional Electrophoresis and Mass Spectrometry
• Manassantin A Isolated from Saururus chinensis Inhibits 5-Lipoxygenase-Dependent Leukotriene C4 Generation by Blocking Mitogen-Activated Protein Kinase Activation in Mast Cells
• Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells
• Involvement of c-Met- and Phosphatidylinositol 3-Kinase Dependent Pathways in Arsenite-Induced Downregulation of Catalase in Hepatoma Cells
• Low Dose Estrogen Supplementation Reduces Mortality of Mice in Estrogen-Dependent Human Tumor Xenograft Model
• Deoxypodophyllotoxin Inhibits the Expression of Intercellular Adhesion Molecule-1 Induced by Tumor Necrosis Factor-.ALPHA. in Murine Lung Epithelial Cells

もっと見る 閉じる

スポンサーリンク