FUNCTIONAL HUMAN HEPATOCYTES : ISOLATION FROM SMALL LIVER BIOPSY SAMPLES AND PRIMARY CULTIVATION WITH LIVER-SPECIFIC FUNCTIONS
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概要
- 論文の詳細を見る
Morphologically and functionally intact human hepatocytes were isolated from small liver biopsy samples weighing about 1-2 g by initial digestion with collagenase followed by repeated digestions with trypsin. The usual yield of hepato-cytes was greater than 1 × 10^7 cells per g of liver sample and cell viability, as judged by dye exclusion test, was routinely over 90%. The isolated human hepatocytes showed intact morphology under scanning electron microscope. Formation of membrane protrusions upon phalloidin addition demonstrated that the actin in isolated hepatocytes was maintained with its structural integrity. The cultured human hepatocytes retained a variety of liver-specific functions which were similarly exhibited by rat hepatocytes isolated using the same procedure. The cultured human hepatocytes exhibited a specific cytochrome P-450 related enzyme activity, and active amino acid uptake that increased upon addition of hormones like glucagon and dexamethasone. Additionally, the cultured human hepatocytes synthesized DNA actively and, human serum albumin, and was found to be responsive to modulation by growth modulating hormones, cytokines and hepatotoxic agents. Based on the profile of activity described above, the presently established conditions for isolation and culturing of human hepatocytes demonstrate that functional liver cells can be obtained from small biopsied liver samples.
- 日本トキシコロジー学会の論文
- 1995-11-25
著者
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KIM Hwan-Mook
Korea Research Institute of Bioscience and Biotechnology
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Cha Young-nam
Medical College Inha University
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HYUN Byung-Hwa
Korea Research Institute of Bioscience and Biotechnology
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OH Goo-Taeg
Korea Research Institute of Bioscience and Biotechnology
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Kim H‐m
Korea Res. Inst. Biosci. And Biotechnol. Taejon Kor
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Han S‐b
Korea Research Institute Of Bioscience And Biotechnology
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HAN Sang-Bae
Korea Research Institute of Bioscience and Biotechnology
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AHN Chang-Joon
Catholic Medical College
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JEONG Kyu-Shik
Korea Research Institute of Bioscience and Biotechnology
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HAN Sang-Bae
College of Pharmacy, Chungbuk National University
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Oh G‐t
Korea Res. Inst. Biosci. And Biotechnol. Taejon Kor
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Han Sang-bae
Korea Research Institute Of Bioscience & Biotechnology
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Kim Hwan-mook
Korea Research Institute Of Bioscience & Biotechnology
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Jeong Kyu
Department Of Veterinary Pathology College Of Veterinary Medicine
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