Antiplatelet Effect of NQ12 : a Possible Mechanism Through the Arachidonic Acid Cascade
スポンサーリンク
概要
- 論文の詳細を見る
NQ12, an antithrombotic agent, has been reported to display a potent antiplatelet activity. This study was undertaken to reveal the effect of NQ12 on rabbit platelet aggregation and signal transduction involved in the arachidonic acid (AA) cascade. NQ12 concentration-dependently suppressed collagen-, AA-, and U46619-induced rabbit platelet aggregation, with IC50 values of 0.71 ± 0.2, 0.82 ± 0.3, and 0.45 ± 0.1 μM, respectively. In addition, the concentration-response curve of U46619 was shifted to the right after NQ12 treatment, indicating an antagonism on thromboxane (TX) A2 receptors. The collagen-stimulated AA liberation was inhibited by NQ12 in the same pattern as its inhibition of platelet aggregation. Further study revealed that NQ12 potently suppressed AA-mediated TXA2 formation, but had no effect on the PGD2 production, indicating an inhibitory effect on TXA2 synthase, which was supported by a TXA2 synthase activity assay indicating that NQ12 concentration-dependently inhibited TXA2 formation converted from PGH2. On the other hand, the AA-stimulated 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) formation was also suppressed by NQ12. Taken together, these results suggest that NQ12 has a potential to inhibit TXA2 synthase activity and TXA2 receptors, and it can modulate AA liberation as well as 12-HETE formation in platelets. This may be a convincing mechanism for the antithrombotic action of NQ12.
- 社団法人 日本薬理学会の論文
- 2007-10-20
著者
-
Lee Jung-jin
Coll. Of Pharmacy Chungnam National Univ.
-
Yun Yeo-Pyo
College of Pharmacy, Chungbuk National University
-
Kim Tack-joong
Div. Of Biological Sci. And Technol. Coll. Of Sci. And Technol. Inst. Of Biomaterials Yonsei Univ.
-
Yun Yeo-pyo
Coll. Of Pharmacy Chungbuk National Univ.
-
Yun Yeo-pyo
College Of Pharmacy Research Center For Bioresource And Health Chungbuk National University
-
Yun Yeo-pyo
College Of Pharmacy Chungbuk National University
-
Yoo Hwan-soo
College Of Pharmacy Cbitrc Chungbuk National University
-
LEE Jung-Jin
College of Pharmacy, Research Center for Bioresource and Health, Chungbuk National University
-
HONG Jin-Tae
College of Pharmacy, Chunghuk National University
-
Lim Yong
Research Institute Of Veterinary Medicine Chungbuk National University
-
Hong Jin
Coll. Of Pharmacy Chungbuk National Univ. Kor
-
Hong Jin-tae
College Of Pharmacy Chunghuk National University
-
Hwang Ji-hye
College Of Pharmacy Cbitrc Chungbuk National University
-
Lee Chong-Kil
College of Pharmacy, Chungbuk National University
-
JIN Yong-Ri
Center for Molecular Medicine, Maine Medical Center Research Institute
-
JIN Yong-Ri
Research Institute of Veterinary Medicine, CBITRC, Chungbuk National University
-
HAN Xiang-Hua
College of Pharmacy, CBITRC, Chungbuk National University
-
LIM Yong
College of Pharmacy, CBITRC, Chungbuk National University
-
KIM Tack-Joong
College of Pharmacy, CBITRC, Chungbuk National University
-
Han Xiang-hua
College Of Pharmacy Cbitrc Chungbuk National University
-
Hong Jin
College Of Pharmacy And Cbitrc Chungbuk National University
-
Lee Chong-kil
Coll. Of Pharmacy Chungbuk National Univ.
-
Jin Yong-ri
Center For Molecular Medicine Maine Medical Center Research Institute
-
Lee Chong-kil
College Of Pharmacy Cbitrc Chungbuk National University
-
Hong Jin-tae
College Of Pharmacy Cbitrc College Of Pharmacy And Medical Research Center Chungbuk National Univers
-
Yun Yeo-pyo
College Of Pharmacy Cbitrc College Of Pharmacy And Medical Research Center Chungbuk National Univers
-
Yun Yeo-pyo
College Of Pharmacy Cbitrc College Of Pharmacy And Medical Research Center Chungbuk National University
-
Hong Jin-Tae
College of Pharmacy and Medical Research Center, Chungbuk National University
-
Lee Jung-Jin
College of Pharmacy, Chungnam National University
-
Yoo Hwan-Soo
College of Pharmacy and Medical Research Center, Chungbuk National University
-
Yun Yeo-Pyo
College of Pharmacy and Medical Research Center, Chungbuk National University
関連論文
- Obovatol Enhances Docetaxel-Induced Prostate and Colon Cancer Cell Death Through Inactivation of Nuclear Transcription Factor-κB
- Inhibitory Effects of Coumarins from the Stem Barks of Fraxinus rhynchophylla on Adipocyte Differentiation in 3T3-L1 Cells
- Potent inhibition of platelet-derived growth factor-stimulated rat aortic vascular smooth muscle cell cycle and proliferation by (2E)-3-(4-hydroxy-3-methoxyphenyl)phenylpro-2-en-1-one, a newly synthesized benzylideneacetophenone derivative
- Potent Inhibition of Platelet-Derived Growth Factor-Stimulated Rat Aortic Vascular Smooth Muscle Cell Cycle and Proliferation by (2E)-3-(4-hydroxy-3-methoxyphenyl)phenylpro-2-en-1-one, a Newly Synthesized Benzylideneacetophenone Derivative
- 2-Hydroxycinnamaldehyde Inhibits SW620 Colon Cancer Cell Growth Through AP-1 Inactivation
- Corynoxeine Isolated from the Hook of Uncaria rhynchophylla Inhibits Rat Aortic Vascular Smooth Muscle Cell Proliferation through the Blocking of Extracellular Signal Regulated Kinase 1/2 Phosphorylation(Pharmacology)
- Inhibition of Cell Growth and Induction of Apoptosis via Inactivation of NF-κB by a Sulfurcompound Isolated From Garlic in Human Colon Cancer Cells
- Protective Effects of Sanjoinine A against N-Methyl-D-aspartate-Induced Seizure(Pharmacology)
- Effects of [5-(2-Methoxy-5-fluorophenyl)furan-2-ylcarbonyl]guanidine (KR-32560), a Novel Sodium/Hydrogen Exchanger-1 Inhibitor, on Myocardial Infarct Size and Ventricular Arrhythmias in a Rat Model of Ischemia/Reperfusion Heart Injury
- Inhibition of Phenotypic and Functional Maturation of Dendritic Cells by Manassantin A
- Sanjoinine A Isolated from Zizyphi Spinosi Semen Augments Pentobarbital-Induced Sleeping Behaviors through the Modification of GABA-ergic Systems(Pharmacology)
- Inhibitory Effects of BST406, a Newly Synthesized Benzylideneacetophenone Derivative, on Abnormal Vascular Smooth Muscle Cell Proliferation
- Genistein Inhibits Rat Aortic Smooth Muscle Cell Proliferation Through the Induction of p27^
- Antiplatelet Effect of NQ12 : a Possible Mechanism Through the Arachidonic Acid Cascade
- Cudratricusxanthone A Isolated from the Root Bark of Cudrania tricuspidata Inhibits the Proliferation of Vascular Smooth Muscle Cells through the Suppression of PDGF-Receptor Beta Tyrosine Kinase(Pharmacology)
- Potent Inhibition of Serum-Stimulated Responses in Vascular Smooth Muscle Cell Proliferation by 2-Chloro-3-(4-hexylphenyl)-amino-1,4-naphthoquinone, a Newly Synthesized 1,4-Naphthoquinone Derivative(Pharmacology)
- ビンクロゾリンの生殖毒性 : 性成熟期雄ラットに対する暴露の影響(毒性学)
- Increase of collagen synthesis by obovatol through stimulation of the TGF-β signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast
- Inhibitory effect of sesaminol glucosides on lipopolysaccharide-induced NF-κB activation and target gene expression in cultured rat astrocytes
- Effect of sesaminol glucosides on β-amyloid-induced PC12 cell death through antioxidant mechanisms
- Anxiolytic-Like Effects of Ginsenosides on the Elevated Plus-Maze Model in Mice(Pharmacology)
- Protective role of Bcl-2 on β-amyloid-induced cell death of differentiated PC12 cells : reduction of NF-κB and p38 MAP kinase activation
- Protective effect of the ethanol extract of Magnolia officinalis and 4-O-methylhonokiol on scopolamine-induced memory impairment and the inhibition of acetylcholinesterase activity
- Inhibitory Effects of Anti-Inflammatory Drugs on Interleukin-6 Bioactivity
- Sauchinone Attenuates Oxidative Stress-Induced Skeletal Muscle Myoblast Damage through the Down-Regulation of Ceramide
- FORMATION OF 8-OXODEOXYGUANOSINE IN LIVER DNA AND HEPATIC INJURY BY PEROXISOME PROLIFERATOR CLOFIBRATE AND PERFLUORODECANOIC ACID IN RATS
- Pharmacological Characterization of Vasorelaxant Effects of BMS-180448, a Novel Cardioselective ATP-Sensitive Potassium Channel Opener, in Rat Aorta
- Inhibitory Effects of Docetaxel on Platelet-Derived Growth Factor (PDGF)-BB–Induced Proliferation of Vascular Smooth Muscle Cells Through Blocking PDGF-Receptor β Phosphorylation
- Antiplatelet and Antithrombotic Activities of NQ301,2-Chloro-3-(4-acetophenyl)-amino-1,4-naphthoquinone
- Inverse Relationship between Adipocyte Differentiation and Ceramide Level in 3T3-L1 Cells
- Antiplatelet Effect of 2-Chloro-3-(4-acetophenyl)-amino-1, 4-naphthoquinone(NQ301) : A Possible Mechanism through Inhibition of Intracellular Ca^Mobilization
- FUMONISIN-INDUCED ELEVATION OF FREE SPHINGANINE AND CELL CONFLUENCY ARE INVERSELY CORRELATED IN LLC-PK CELLS
- α-Viniferin Suppresses the Signal Transducer and Activation of Transcription-1 (STAT-1)-Inducible Inflammatory Genes in Interferon-γ-Stimulated Macrophages
- Inhibitory Effects of Docetaxel on Platelet-Derived Growth Factor (PDGF)-BB-Induced Proliferation of Vascular Smooth Muscle Cells Through Blocking PDGF-Receptor β Phosphorylation
- Clitocybin A, a Novel Isoindolinone, From the Mushroom Clitocybe aurantiaca, Inhibits Cell Proliferation Through G1 Phase Arrest by Regulating the PI3K/Akt Cascade in Vascular Smooth Muscle Cells
- Inhibitory Effect of Eriodictyol on IgE/Ag-Induced Type I Hypersensitivity
- Protection of Apigenin against Kainate-Induced Excitotoxicity by Anti-oxidative Effects
- JJK694, a Synthesized Obovatol Derivative, Inhibits Platelet Activation by Suppressing Cyclooxygenase and Lipoxygenase Activities