Inhibitory Effects of Coumarins from the Stem Barks of Fraxinus rhynchophylla on Adipocyte Differentiation in 3T3-L1 Cells
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概要
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In the course of screening anti-adipogenic activity of natural products employing the preadipocyte cell line, 3T3-L1 as an in vitro assay system, the EtOAc fraction of the stem barks of Fraxinus rhynchophylla DENCE (Oleaceae) showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Activity-guided fractionation led to the isolation of six coumarins such as esculetin (1), scopoletin (2), fraxetin (3), fraxidin (4) esculin (5) and fraxin (6). Among the six coumarins isolated, esculetin (1) showed the most potent inhibitory activity on adipocyte differentiation, followed by fraxetin (3). Further studies with interval treatment demonstrated that esculetin (1) exerted inhibitory activity on adipocyte differentiation when treated within 2 d (days 0—2) after differentiation induction. We further investigated the effect of esculetin (1) on peroxisome proliferator activated receptor γ (PPARγ), one of the early adipogenic transcription factors. Esculetin (1) significantly blocked the induction of PPARγ protein expression and inhibited adipocyte differentiation induced by troglitazone, a PPARγ agonist. Taken together, these results suggest that esculetin (1), an active compound from F. rhynchophylla, inhibited early stage of adipogenic differentiation, in part, via inhibition of PPARγ-dependent pathway.
著者
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Lee Mi
College Of Veterinary Medicine Chonbuk National University
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Hwang Bang
College Of Pharmacy Chungbuk National University
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Yoo Hwan-soo
College Of Pharmacy Cbitrc Chungbuk National University
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Shin Eunjin
College of Pharmacy, Chungbuk National University
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Choi Kyeong-Mi
College of Pharmacy, Chungbuk National University
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Lee Chong-Kil
College of Pharmacy, Chungbuk National University
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Lee Chong-kil
College Of Pharmacy Cbitrc Chungbuk National University
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Lee Mi
College Of Pharmacy Chungbuk National University
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Shin Eunjin
College Of Pharmacy Chungbuk National University
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Lee Mi
College of Pharmacy and Medical Research Center, Chungbuk National University
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Lee Mi
College of Pharmacy, Chungbuk National University
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Choi Kyeong-Mi
College of Pharmacy and Medical Research Center, Chungbuk National University
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Yoo Hwan-Soo
College of Pharmacy and Medical Research Center, Chungbuk National University
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- Inhibitory Effects of Coumarins from the Stem Barks of Fraxinus rhynchophylla on Adipocyte Differentiation in 3T3-L1 Cells
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