Non-Reducing Terminal Fucose within N-Linked Glycan Plays a Significant Role in the Recognition of Human Milk Lactoferrin by the 1CF11 Monoclonal Antibody

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概要

• 論文の詳細を見る

• We have recently demonstrated that the 1CF11 monoclonal antibody bound human milk lactoferrin (hLf) through the recognition of two distinct portions of the molecule, namely the N-glycan-relevant and -irrelevant structural elements. In this present study, we prepared four immunoreactive peptide fractions containing N-linked glycan from tryptic digests of reduced and alkylated hLf by using a concanavalin A lectin column and reverse-phase HPLC. Deglycosylation of these fractions and a competitive binding assay using fucosylated oligosaccharides revealed that the non-reducing terminal fucose residue in N-linked glycan(s) played a significant role in recognizing the N-glycan-relevant element in hLf by 1CF11.

• 社団法人 日本農芸化学会の論文
• 2010-10-23

著者

• 金丸 美敬

  Dep. Of Applied Life Sci. Gifu Univ.

• Kanamaru Yoshihiro

  Department Of Food Science Faculty Of Agriculture Gifu University

• Kanamaru Yoshihiro

  Department Of Food Science Faculty Of Applied Biological Science Gifu University

• Kanamaru Yoshihiro

  Department Of Applied Life Science Faculty Of Applied Biological Sciences Gifu University

• Kanamaru Yoshihiro

  Faculty Of Applied Biological Sciences Gifu University

• YABE Tomio

  Department of Applied Life Science, Gifu University

• Fukaya Shinichi

  The United Graduate School Of Agricultural Science Gifu University

• FUKAYA Shinichi

  United Graduate School of Agricultural Science, Gifu University

• Yabe Tomio

  Department Of Applied Life Science Gifu University

• Yabe Tomio

  United Graduate School of Agricultural Science, Gifu University

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